Discovery of widespread GTP-binding motifs in genomic DNA and RNA
Curtis, Edward A.
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CitationCurtis, Edward A., and David R. Liu. 2013. Discovery of widespread GTP-binding motifs in genomic DNA and RNA. Chemistry & Biology 20, no. 4: 521–532. doi:10.1016/j.chembiol.2013.02.015.
AbstractBiological RNAs that bind small-molecules have been implicated in a variety of regulatory and catalytic processes. Inspired by these examples, we used in vitro selection to search a pool of genomeencoded RNA fragments for naturally occurring GTP aptamers. Several classes of aptamers were identified, including one ("the G motif") with a G-quadruplex structure. Further analysis revealed that most RNA and DNA G-quadruplexes bind GTP. The G motif is abundant in eukaryotes, and the human genome contains ∼75,000 examples with dissociation constants comparable to the GTP concentration of a eukaryotic cell (∼300 μM). G-quadruplexes play roles in diverse cellular processes, and our findings raise the possibility that GTP may play a role in the function of these elements. Consistent with this possibility, the sequence requirements of several classes of regulatory G-quadruplexes parallel those of GTP binding.
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