Discovery and biological characterization of geranylated RNA in bacteria

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Discovery and biological characterization of geranylated RNA in bacteria

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Title: Discovery and biological characterization of geranylated RNA in bacteria
Author: Dumelin, Christoph; Chen, Yiyun; Leconte, Aaron M; Chen, Y Grace; Liu, David Ruchien

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Citation: Dumelin, Christoph E, Yiyun Chen, Aaron M Leconte, Y Grace Chen, and David R Liu. 2012. Discovery and biological characterization of geranylated RNA in bacteria. Nature Chemical Biology 8, no. 11: 913-919. doi:10.1038/nchembio.1070.
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Abstract: A general mass spectrometry-based screen for unusually hydrophobic cellular small-molecule RNA conjugates revealed geranylated RNA in E. coli, Enterobacter aerogenes, Pseudomonas aeruginosa, and Salmonella thyphimurium. The geranyl group is conjugated to the sulfur atom in two 5-methylaminomethyl-2-thiouridine nucleotides. These geranylated nucleotides occur in the first anticodon position of tRNA Glu UUC, tRNA Lys UUU, tRNA Gln UUG at a frequency of up to 6.7% (~400 geranylated nucleotides per cell). RNA geranylation levels can be increased or abolished by mutation or deletion of the selU (ybbB) gene in E. coli, and purified SelU protein in the presence of geranyl pyrophosphate and tRNA can produce geranylated tRNA. The presence or absence of the geranyl group in tRNA Glu UUC, tRNA Lys UUU, and tRNA Gln UUG affects codon bias and frameshifting during translation. These RNAs represent the first reported examples of oligoisoprenylated cellular nucleic acids.
Published Version: doi:10.1038/nchembio.1070
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3494293/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:27409157
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