LC/MS analysis of cellular RNA reveals NAD-linked RNA

 
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Chen, Y. Grace
Shen, Yinghua
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https://doi.org/10.1038/nchembio.235
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Chen, Y Grace, Walter E Kowtoniuk, Isha Agarwal, Yinghua Shen, and David R Liu. 2009. LC/MS analysis of cellular RNA reveals NAD-linked RNA. Nature Chemical Biology 5, no. 12: 879–881. doi:10.1038/nchembio.235.
Abstract
We developed a general method to detect cellular small molecule-RNA conjugates that does not rely on the reactivity of the small molecule, revealing NAD-linked RNA in E. coli and S. venezuelae. Subsequent characterization shows NAD is a 5’ modification of RNA, cannot be installed in vitro through aberrant transcriptional initiation, is only found among smaller cellular RNAs, and is present at a surprisingly high abundance of ~3000 copies per cell.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2842606/
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http://nrs.harvard.edu/urn-3:HUL.InstRepos:27409160

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