Discovery and characterization of a peptide that enhances endosomal escape of delivered proteins in vitro and in vivo
LaRochelle, Jonathan R.
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CitationLi, Margie, Yong Tao, Yilai Shu, Jonathan R. LaRochelle, Angela Steinauer, David Thompson, Alanna Schepartz, Zheng-Yi Chen, and David R. Liu. 2015. Discovery and characterization of a peptide that enhances endosomal escape of delivered proteins in vitro and in vivo. Journal of the American Chemical Society 137, no. 44: 14084–14093. doi:10.1021/jacs.5b05694.
AbstractThe in efficient delivery of proteins into mammalian cells remains a major barrier to realizing the therapeutic potential of many proteins. We and others have previously shown that superpositively charged proteins are efficiently endocytosed and can bring associated proteins and nucleic acids into cells. The vast majority of cargo delivered in this manner, however, remains in endosomes and does not reach the cytosol. In this study we designed and implemented a screen to discover peptides that enhance the endosomal escape of proteins fused to superpositively charged GFP (+36 GFP). From a screen of peptides previously reported to disrupt microbial membranes without known mammalian cell toxicity, we discovered a 13-residue peptide, aurein 1.2, that substantially increases cytosolic protein delivery by up to ~5-fold in a cytosolic fractionation assay in cultured cells. Four additional independent assays for non-endosomal protein delivery collectively suggest that aurein 1.2 enhances endosomal escape of associated endocytosed protein cargo. Structure-function studies clarified peptide sequence and protein conjugation requirements for endosomal escape activity. When applied to the in vivo delivery of +36 GFP–Crerecombinase fusions into the inner ear of live mice, fusion with aurein 1.2 dramatically increased non-endosomal Crerecombinase delivery potency, resulting in up to 100% recombined inner hair cellsand 96% recombined outer hair cells, compared to 0-4% recombined hair cells from +36-GFP- Cre without aurein 1.2. Collectively, these findings describe a genetically encodable, endosome escape-enhancing peptide that can substantially increase the cytoplasmic delivery of cationic proteins
in vitro and in vivo.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:27413829
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