MicroRNA-Mediated Control of Cell Fate in Megakaryocyte-Erythrocyte Progenitors
Wang, Judy Y.
Dombkowski, David M.
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CitationLu, Jun, Shangqin Guo, Benjamin L. Ebert, Hao Zhang, Xiao Peng, Jocelyn Bosco, Jennifer Pretz, et al. 2008. “MicroRNA-Mediated Control of Cell Fate in Megakaryocyte-Erythrocyte Progenitors.” Developmental Cell 14, no. 6: 843–853. doi:10.1016/j.devcel.2008.03.012.
AbstractLineage specification is a critical issue in developmental and regenerative biology. We hypothesized that microRNAs (miRNAs) are important participants in that process and used the poorly-understood regulation of megakaryocyte-erythrocyte progenitors (MEPs) in hematopoiesis as a model system. We report here that miR-150 modulates lineage fate in MEPs. Using a novel methodology capable of profiling miRNA expression in limiting numbers of primary cells, we identify miR-150 as preferentially expressed in the megakaryocytic lineage. Through gain- and loss-of-function experiments, we demonstrate that miR-150 drives MEP differentiation toward megakaryocytes at the expense of erythroid cells in vitro and in vivo. Moreover, we identify the transcription factor MYB as a critical target of miR-150 in this regulation. These experiments show that miR-150 regulates MEP fate, and thus establish a role for miRNAs in lineage specification of mammalian multi-potent cells.
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