dc.contributor.author | Lu, Jun | |
dc.contributor.author | Guo, Shangqin | |
dc.contributor.author | Ebert, Benjamin L. | |
dc.contributor.author | Zhang, Hao | |
dc.contributor.author | Peng, Xiao | |
dc.contributor.author | Bosco, Jocelyn | |
dc.contributor.author | Pretz, Jennifer Lee | |
dc.contributor.author | Schlanger, Rita | |
dc.contributor.author | Wang, Judy Y. | |
dc.contributor.author | Mak, Raymond Heungwing | |
dc.contributor.author | Dombkowski, David M. | |
dc.contributor.author | Preffer, Frederic Ira | |
dc.contributor.author | Scadden, David Thomas | |
dc.contributor.author | Golub, Todd Robert | |
dc.date.accessioned | 2016-07-11T18:01:33Z | |
dc.date.issued | 2008 | |
dc.identifier.citation | Lu, Jun, Shangqin Guo, Benjamin L. Ebert, Hao Zhang, Xiao Peng, Jocelyn Bosco, Jennifer Pretz, et al. 2008. “MicroRNA-Mediated Control of Cell Fate in Megakaryocyte-Erythrocyte Progenitors.” Developmental Cell 14, no. 6: 843–853. doi:10.1016/j.devcel.2008.03.012. | en_US |
dc.identifier.issn | 1534-5807 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:27527730 | |
dc.description.abstract | Lineage specification is a critical issue in developmental and regenerative biology. We hypothesized that microRNAs (miRNAs) are important participants in that process and used the poorly-understood regulation of megakaryocyte-erythrocyte progenitors (MEPs) in hematopoiesis as a model system. We report here that miR-150 modulates lineage fate in MEPs. Using a novel methodology capable of profiling miRNA expression in limiting numbers of primary cells, we identify miR-150 as preferentially expressed in the megakaryocytic lineage. Through gain- and loss-of-function experiments, we demonstrate that miR-150 drives MEP differentiation toward megakaryocytes at the expense of erythroid cells in vitro and in vivo. Moreover, we identify the transcription factor MYB as a critical target of miR-150 in this regulation. These experiments show that miR-150 regulates MEP fate, and thus establish a role for miRNAs in lineage specification of mammalian multi-potent cells. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier BV | en_US |
dc.relation.isversionof | doi:10.1016/j.devcel.2008.03.012 | en_US |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2688789/ | en_US |
dash.license | LAA | |
dc.title | MicroRNA-Mediated Control of Cell Fate in Megakaryocyte-Erythrocyte Progenitors | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Accepted Manuscript | en_US |
dc.relation.journal | Developmental Cell | en_US |
dash.depositing.author | Preffer, Frederic Ira | |
dc.date.available | 2016-07-11T18:01:33Z | |
dc.identifier.doi | 10.1016/j.devcel.2008.03.012 | * |
dash.authorsordered | false | |
dash.contributor.affiliated | Lu, Jun | |
dash.contributor.affiliated | Preffer, Frederic | |
dash.contributor.affiliated | Pretz, Jennifer | |
dash.contributor.affiliated | Mak, Raymond | |
dash.contributor.affiliated | Golub, Todd | |
dash.contributor.affiliated | Ebert, Benjamin | |
dash.contributor.affiliated | Scadden, David | |