Show simple item record

dc.contributor.authorFame, Ryan Marie
dc.contributor.authorMacDonald, Jessica Linn
dc.contributor.authorDunwoodie, S. L.
dc.contributor.authorOki, Emi Takahashi
dc.contributor.authorMacklis, Jeffrey Daniel
dc.date.accessioned2016-07-11T18:10:25Z
dc.date.issued2016
dc.identifierQuick submit: 2016-07-05T10:12:47-0400
dc.identifier.citationFame, R. M., J. L. MacDonald, S. L. Dunwoodie, E. Takahashi, and J. D. Macklis. 2016. “Cited2 Regulates Neocortical Layer II/III Generation and Somatosensory Callosal Projection Neuron Development and Connectivity.” Journal of Neuroscience 36 (24) (June 15): 6403–6419. doi:10.1523/jneurosci.4067-15.2016.en_US
dc.identifier.issn0270-6474en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:27531159
dc.description.abstractThe neocortex contains hundreds to thousands of distinct subtypes of precisely connected neurons, allowing it to perform remarkably complex tasks of high-level cognition. Callosal projection neurons (CPN) connect the cerebral hemispheres via the corpus callosum, integrating cortical information and playing key roles in associative cognition. CPN are a strikingly diverse set of neuronal subpopulations, and development of this diversity requires precise control by a complex, interactive set of molecular effectors. We have found that the transcriptional coregulator Cited2 regulates and refines two stages of CPN development. Cited2 is expressed broadly by progenitors in the embryonic day 15.5 subventricular zone, during the peak of superficial layer CPN birth, with a progressive postmitotic refinement in expression, becoming restricted to CPN of the somatosensory cortex postnatally. We generated progenitor-stage and postmitotic forebrain-specific Cited2 conditional knock-out mice, using the Emx1-Cre and NEX-Cre mouse lines, respectively. We demonstrate that Cited2 functions in progenitors, but is not necessary postmitotically, to regulate both (1) broad generation of layer II/III CPN and (2) acquisition of precise area-specific molecular identity and axonal/dendritic connectivity of somatosensory CPN. This novel CPN subtype-specific and area-specific control from progenitor action of Cited2 adds yet another layer of complexity to the multistage developmental regulation of neocortical development.en_US
dc.description.sponsorshipStem Cell and Regenerative Biologyen_US
dc.language.isoen_USen_US
dc.publisherSociety for Neuroscienceen_US
dc.relation.isversionof10.1523/jneurosci.4067-15.2016en_US
dash.licenseOAP
dc.subjectarealizationen_US
dc.subjectcallosumen_US
dc.subjectneocortexen_US
dc.subjectneuronal differentiationen_US
dc.subjectsomatosensoryen_US
dc.titleCited2 Regulates Neocortical Layer II/III Generation and Somatosensory Callosal Projection Neuron Development and Connectivityen_US
dc.typeJournal Articleen_US
dc.date.updated2016-07-05T14:12:53Z
dc.description.versionAccepted Manuscripten_US
dc.relation.journalJournal of Neuroscienceen_US
dash.depositing.authorMacklis, Jeffrey Daniel
dc.date.available2016
dc.date.available2016-07-11T18:10:25Z
dash.funder.nameNIHen_US
dash.funder.awardNS41590en_US
dash.funder.awardNS093376en_US
dash.funder.awardNS45523en_US
dash.funder.awardNS49553en_US
dash.funder.awardNS075672en_US
dash.funder.awardF31 NS073163en_US
dash.funder.awardNIH R01HD078561en_US
dash.funder.awardR21HD069001en_US
dash.funder.awardID1042002en_US
dash.funder.awardR37 NS41590en_US
dc.identifier.doi10.1523/jneurosci.4067-15.2016*
dash.authorsorderedfalse
dash.contributor.affiliatedOki, Emi
dash.contributor.affiliatedFame, Ryan
dash.contributor.affiliatedMacDonald, Jessica Linn
dash.contributor.affiliatedMacklis, Jeffrey


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record