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dc.contributor.authorLutas, Andrewen_US
dc.contributor.authorLahmann, Carolinaen_US
dc.contributor.authorSoumillon, Magalien_US
dc.contributor.authorYellen, Garyen_US
dc.date.accessioned2016-07-14T19:11:53Z
dc.date.issued2016en_US
dc.identifier.citationLutas, Andrew, Carolina Lahmann, Magali Soumillon, and Gary Yellen. 2016. “The leak channel NALCN controls tonic firing and glycolytic sensitivity of substantia nigra pars reticulata neurons.” eLife 5 (1): e15271. doi:10.7554/eLife.15271. http://dx.doi.org/10.7554/eLife.15271.en
dc.identifier.issn2050-084Xen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:27662040
dc.description.abstractCertain neuron types fire spontaneously at high rates, an ability that is crucial for their function in brain circuits. The spontaneously active GABAergic neurons of the substantia nigra pars reticulata (SNr), a major output of the basal ganglia, provide tonic inhibition of downstream brain areas. A depolarizing 'leak' current supports this firing pattern, but its molecular basis remains poorly understood. To understand how SNr neurons maintain tonic activity, we used single-cell RNA sequencing to determine the transcriptome of individual mouse SNr neurons. We discovered that SNr neurons express the sodium leak channel, NALCN, and that SNr neurons lacking NALCN have impaired spontaneous firing. In addition, NALCN is involved in the modulation of excitability by changes in glycolysis and by activation of muscarinic acetylcholine receptors. Our findings suggest that disruption of NALCN could impair the basal ganglia circuit, which may underlie the severe motor deficits in humans carrying mutations in NALCN. DOI: http://dx.doi.org/10.7554/eLife.15271.001en
dc.language.isoen_USen
dc.publishereLife Sciences Publications, Ltden
dc.relation.isversionofdoi:10.7554/eLife.15271en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4902561/pdf/en
dash.licenseLAAen_US
dc.subjectspontaneous firingen
dc.subjectbasal gangliaen
dc.subjectleak currenten
dc.subjectmetabolism and excitabilityen
dc.subjectMouseen
dc.titleThe leak channel NALCN controls tonic firing and glycolytic sensitivity of substantia nigra pars reticulata neuronsen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journaleLifeen
dash.depositing.authorLutas, Andrewen_US
dc.date.available2016-07-14T19:11:53Z
dc.identifier.doi10.7554/eLife.15271*
dash.contributor.affiliatedLutas, Andrew
dash.contributor.affiliatedLahmann, Carolina
dash.contributor.affiliatedYellen, Gary


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