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dc.contributor.authorMeyer, Michaelaen_US
dc.contributor.authorDhamne, Sameer C.en_US
dc.contributor.authorLaCoursiere, Christopher M.en_US
dc.contributor.authorTambunan, Dimiraen_US
dc.contributor.authorPoduri, Annapurnaen_US
dc.contributor.authorRotenberg, Alexanderen_US
dc.date.accessioned2016-07-14T19:13:46Z
dc.date.issued2016en_US
dc.identifier.citationMeyer, Michaela, Sameer C. Dhamne, Christopher M. LaCoursiere, Dimira Tambunan, Annapurna Poduri, and Alexander Rotenberg. 2016. “Microarray Noninvasive Neuronal Seizure Recordings from Intact Larval Zebrafish.” PLoS ONE 11 (6): e0156498. doi:10.1371/journal.pone.0156498. http://dx.doi.org/10.1371/journal.pone.0156498.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:27662099
dc.description.abstractZebrafish epilepsy models are emerging tools in experimental epilepsy. Zebrafish larvae, in particular, are advantageous because they can be easily genetically altered and used for developmental and drug studies since agents applied to the bath penetrate the organism easily. Methods for electrophysiological recordings in zebrafish are new and evolving. We present a novel multi-electrode array method to non-invasively record electrical activity from up to 61 locations of an intact larval zebrafish head. This method enables transcranial noninvasive recording of extracellular field potentials (which include multi-unit activity and EEG) to identify epileptic seizures. To record from the brains of zebrafish larvae, the dorsum of the head of an intact larva was secured onto a multi-electrode array. We recorded from individual electrodes for at least three hours and quantified neuronal firing frequency, spike patterns (continuous or bursting), and synchrony of neuronal firing. Following 15 mM potassium chloride- or pentylenetetrazole-infusion into the bath, spike and burst rate increased significantly. Additionally, synchrony of neuronal firing across channels, a hallmark of epileptic seizures, also increased. Notably, the fish survived the experiment. This non-invasive method complements present invasive zebrafish neurophysiological techniques: it affords the advantages of high spatial and temporal resolution, a capacity to measure multiregional activity and neuronal synchrony in seizures, and fish survival for future experiments, such as studies of epileptogenesis and development.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0156498en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900632/pdf/en
dash.licenseLAAen_US
dc.subjectBiology and Life Sciencesen
dc.subjectPhysiologyen
dc.subjectElectrophysiologyen
dc.subjectMembrane Potentialen
dc.subjectAction Potentialsen
dc.subjectMedicine and Health Sciencesen
dc.subjectNeurophysiologyen
dc.subjectNeuroscienceen
dc.subjectDevelopmental Biologyen
dc.subjectMetamorphosisen
dc.subjectLarvaeen
dc.subjectModel Organismsen
dc.subjectAnimal Modelsen
dc.subjectZebrafishen
dc.subjectOrganismsen
dc.subjectAnimalsen
dc.subjectVertebratesen
dc.subjectFishesen
dc.subjectOsteichthyesen
dc.subjectNeurologyen
dc.subjectEpilepsyen
dc.subjectBioassays and Physiological Analysisen
dc.subjectElectrophysiological Techniquesen
dc.subjectBrain Electrophysiologyen
dc.subjectElectroencephalographyen
dc.subjectBrain Mappingen
dc.subjectDiagnostic Medicineen
dc.subjectClinical Neurophysiologyen
dc.subjectImaging Techniquesen
dc.subjectNeuroimagingen
dc.subjectMembrane Electrophysiologyen
dc.subjectElectrode Recordingen
dc.subjectEpileptic Seizuresen
dc.titleMicroarray Noninvasive Neuronal Seizure Recordings from Intact Larval Zebrafishen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dash.depositing.authorPoduri, Annapurnaen_US
dc.date.available2016-07-14T19:13:46Z
dc.identifier.doi10.1371/journal.pone.0156498*
dash.contributor.affiliatedPoduri, Annapurna
dash.contributor.affiliatedRotenberg, Alexander


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