“Deadman” and “Passcode” microbial kill switches for bacterial containment
Chan, Clement T. Y.
Lee, Jeong Wook
Cameron, D. Ewen
Bashor, Caleb J.
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CitationChan, Clement T. Y., Jeong Wook Lee, D. Ewen Cameron, Caleb J. Bashor, and James J. Collins. 2015. ““Deadman” and “Passcode” microbial kill switches for bacterial containment.” Nature chemical biology 12 (2): 82-86. doi:10.1038/nchembio.1979. http://dx.doi.org/10.1038/nchembio.1979.
AbstractBiocontainment systems that couple environmental sensing with circuit-based control of cell viability could be used to prevent escape of genetically modified microbes into the environment. Here we present two engineered safe-guard systems: the Deadman and Passcode kill switches. The Deadman kill switch uses unbalanced reciprocal transcriptional repression to couple a specific input signal with cell survival. The Passcode kill switch uses a similar two-layered transcription design and incorporates hybrid LacI/GalR family transcription factors to provide diverse and complex environmental inputs to control circuit function. These synthetic gene circuits efficiently kill Escherichia coli and can be readily reprogrammed to change their environmental inputs, regulatory architecture and killing mechanism.
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