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dc.contributor.authorChan, Clement T. Y.en_US
dc.contributor.authorLee, Jeong Wooken_US
dc.contributor.authorCameron, D. Ewenen_US
dc.contributor.authorBashor, Caleb J.en_US
dc.contributor.authorCollins, James J.en_US
dc.date.accessioned2016-07-14T19:14:18Z
dc.date.issued2015en_US
dc.identifier.citationChan, Clement T. Y., Jeong Wook Lee, D. Ewen Cameron, Caleb J. Bashor, and James J. Collins. 2015. ““Deadman” and “Passcode” microbial kill switches for bacterial containment.” Nature chemical biology 12 (2): 82-86. doi:10.1038/nchembio.1979. http://dx.doi.org/10.1038/nchembio.1979.en
dc.identifier.issn1552-4450en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:27662119
dc.description.abstractBiocontainment systems that couple environmental sensing with circuit-based control of cell viability could be used to prevent escape of genetically modified microbes into the environment. Here we present two engineered safe-guard systems: the Deadman and Passcode kill switches. The Deadman kill switch uses unbalanced reciprocal transcriptional repression to couple a specific input signal with cell survival. The Passcode kill switch uses a similar two-layered transcription design and incorporates hybrid LacI/GalR family transcription factors to provide diverse and complex environmental inputs to control circuit function. These synthetic gene circuits efficiently kill Escherichia coli and can be readily reprogrammed to change their environmental inputs, regulatory architecture and killing mechanism.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.1038/nchembio.1979en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4718764/pdf/en
dash.licenseLAAen_US
dc.title“Deadman” and “Passcode” microbial kill switches for bacterial containmenten
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNature chemical biologyen
dash.depositing.authorCollins, James J.en_US
dc.date.available2016-07-14T19:14:18Z
dc.identifier.doi10.1038/nchembio.1979*
dash.contributor.affiliatedCollins, James


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