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dc.contributor.authorLong, Jingen_US
dc.contributor.authorBasu Roy, Robindraen_US
dc.contributor.authorZhang, Yanjia J.en_US
dc.contributor.authorAntrobus, Robinen_US
dc.contributor.authorDu, Yuxianen_US
dc.contributor.authorSmith, Duncan L.en_US
dc.contributor.authorWeekes, Michael P.en_US
dc.contributor.authorJavid, Babaken_US
dc.date.accessioned2016-08-09T14:52:07Z
dc.date.issued2016en_US
dc.identifier.citationLong, Jing, Robindra Basu Roy, Yanjia J. Zhang, Robin Antrobus, Yuxian Du, Duncan L. Smith, Michael P. Weekes, and Babak Javid. 2016. “Plasma Membrane Profiling Reveals Upregulation of ABCA1 by Infected Macrophages Leading to Restriction of Mycobacterial Growth.” Frontiers in Microbiology 7 (1): 1086. doi:10.3389/fmicb.2016.01086. http://dx.doi.org/10.3389/fmicb.2016.01086.en
dc.identifier.issn1664-302Xen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:27822079
dc.description.abstractThe plasma membrane represents a critical interface between the internal and extracellular environments, and harbors multiple proteins key receptors and transporters that play important roles in restriction of intracellular infection. We applied plasma membrane profiling, a technique that combines quantitative mass spectrometry with selective cell surface aminooxy-biotinylation, to Bacille Calmette–Guérin (BCG)-infected THP-1 macrophages. We quantified 559 PM proteins in BCG-infected THP-1 cells. One significantly upregulated cell-surface protein was the cholesterol transporter ABCA1. We showed that ABCA1 was upregulated on the macrophage cell-surface following infection with pathogenic mycobacteria and knockdown of ABCA1 resulted in increased mycobacterial survival within macrophages, suggesting that it may be a novel mycobacterial host-restriction factor.en
dc.language.isoen_USen
dc.publisherFrontiers Media S.A.en
dc.relation.isversionofdoi:10.3389/fmicb.2016.01086en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4940386/pdf/en
dash.licenseLAAen_US
dc.subjectplasma membrane profilingen
dc.subjecten
dc.subjectABCA1en
dc.subjectcholesterolen
dc.subjectSILACen
dc.titlePlasma Membrane Profiling Reveals Upregulation of ABCA1 by Infected Macrophages Leading to Restriction of Mycobacterial Growthen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalFrontiers in Microbiologyen
dash.depositing.authorZhang, Yanjia J.en_US
dc.date.available2016-08-09T14:52:07Z
dc.identifier.doi10.3389/fmicb.2016.01086*
dash.contributor.affiliatedZhang, Yanjia


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