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dc.contributor.authorTakatani, Tomozumien_US
dc.contributor.authorShirakawa, Junen_US
dc.contributor.authorRoe, Michael W.en_US
dc.contributor.authorLeech, Colin A.en_US
dc.contributor.authorMaier, Bernhard F.en_US
dc.contributor.authorMirmira, Raghavendra G.en_US
dc.contributor.authorKulkarni, Rohit N.en_US
dc.date.accessioned2016-08-09T14:52:08Z
dc.date.issued2016en_US
dc.identifier.citationTakatani, Tomozumi, Jun Shirakawa, Michael W. Roe, Colin A. Leech, Bernhard F. Maier, Raghavendra G. Mirmira, and Rohit N. Kulkarni. 2016. “IRS1 deficiency protects β-cells against ER stress-induced apoptosis by modulating sXBP-1 stability and protein translation.” Scientific Reports 6 (1): 28177. doi:10.1038/srep28177. http://dx.doi.org/10.1038/srep28177.en
dc.identifier.issn2045-2322en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:27822082
dc.description.abstractEndoplasmic reticulum (ER) stress is among several pathological features that underlie β-cell failure in the development of type 1 and type 2 diabetes. Adaptor proteins in the insulin/insulin-like-growth factor-1 signaling pathways, such as insulin receptor substrate-1 (IRS1) and IRS2, differentially impact β-cell survival but the underlying mechanisms remain unclear. Here we report that β-cells deficient in IRS1 (IRS1KO) are resistant, while IRS2 deficiency (IRS2KO) makes them susceptible to ER stress-mediated apoptosis. IRS1KOs exhibited low nuclear accumulation of spliced XBP-1 due to its poor stability, in contrast to elevated accumulation in IRS2KO. The reduced nuclear accumulation in IRS1KO was due to protein instability of Xbp1 secondary to proteasomal degradation. IRS1KO also demonstrated an attenuation in their general translation status in response to ER stress revealed by polyribosomal profiling. Phosphorylation of eEF2 was dramatically increased in IRS1KO enabling the β-cells to adapt to ER stress by blocking translation. Furthermore, significantly high ER calcium (Ca2+) was detected in IRS1KO β-cells even upon induction of ER stress. These observations suggest that IRS1 could be a therapeutic target for β-cell protection against ER stress-mediated cell death by modulating XBP-1 stability, protein synthesis, and Ca2+ storage in the ER.en
dc.language.isoen_USen
dc.publisherNature Publishing Groupen
dc.relation.isversionofdoi:10.1038/srep28177en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4932502/pdf/en
dash.licenseLAAen_US
dc.titleIRS1 deficiency protects β-cells against ER stress-induced apoptosis by modulating sXBP-1 stability and protein translationen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalScientific Reportsen
dash.depositing.authorShirakawa, Junen_US
dc.date.available2016-08-09T14:52:08Z
dc.identifier.doi10.1038/srep28177*
dash.contributor.affiliatedShirakawa, Jun
dash.contributor.affiliatedKulkarni, Rohit


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