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dc.contributor.authorPatrick, Alisonen_US
dc.contributor.authorSeluanov, Michaelen_US
dc.contributor.authorHwang, Chaewonen_US
dc.contributor.authorTam, Jonathanen_US
dc.contributor.authorKhan, Tanyaen_US
dc.contributor.authorMorgenstern, Arien_US
dc.contributor.authorWiener, Laurenen_US
dc.contributor.authorVazquez, Juan M.en_US
dc.contributor.authorZafar, Hibaen_US
dc.contributor.authorWen, Roberten_US
dc.contributor.authorMuratkalyeva, Malikaen_US
dc.contributor.authorDoerig, Katherineen_US
dc.contributor.authorZagorulya, Mariaen_US
dc.contributor.authorCole, Laurenen_US
dc.contributor.authorCatalano, Sophiaen_US
dc.contributor.authorLobo Ladd, Aliny ABen_US
dc.contributor.authorCoppi, A. Augustoen_US
dc.contributor.authorCoşkun, Yükselen_US
dc.contributor.authorTian, Xiaoen_US
dc.contributor.authorAblaeva, Juliaen_US
dc.contributor.authorNevo, Eviataren_US
dc.contributor.authorGladyshev, Vadim N.en_US
dc.contributor.authorZhang, Zhengdong D.en_US
dc.contributor.authorVijg, Janen_US
dc.contributor.authorSeluanov, Andreien_US
dc.contributor.authorGorbunova, Veraen_US
dc.date.accessioned2016-08-09T14:52:59Z
dc.date.issued2016en_US
dc.identifier.citationPatrick, A., M. Seluanov, C. Hwang, J. Tam, T. Khan, A. Morgenstern, L. Wiener, et al. 2016. “Sensitivity of primary fibroblasts in culture to atmospheric oxygen does not correlate with species lifespan.” Aging (Albany NY) 8 (5): 841-847. doi:10.18632/aging.100958. http://dx.doi.org/10.18632/aging.100958.en
dc.identifier.issn1945-4589en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:27822223
dc.description.abstractDifferences in the way human and mouse fibroblasts experience senescence in culture had long puzzled researchers. While senescence of human cells is mediated by telomere shortening, Parrinello et al. demonstrated that senescence of mouse cells is caused by extreme oxygen sensitivity. It was hypothesized that the striking difference in oxygen sensitivity between mouse and human cells explains their different rates of aging. To test if this hypothesis is broadly applicable, we cultured cells from 16 rodent species with diverse lifespans in 3% and 21% oxygen and compared their growth rates. Unexpectedly, fibroblasts derived from laboratory mouse strains were the only cells demonstrating extreme sensitivity to oxygen. Cells from hamster, muskrat, woodchuck, capybara, blind mole rat, paca, squirrel, beaver, naked mole rat and wild-caught mice were mildly sensitive to oxygen, while cells from rat, gerbil, deer mouse, chipmunk, guinea pig and chinchilla showed no difference in the growth rate between 3% and 21% oxygen. We conclude that, although the growth of primary fibroblasts is generally improved by maintaining cells in 3% oxygen, the extreme oxygen sensitivity is a peculiarity of laboratory mouse strains, possibly related to their very long telomeres, and fibroblast oxygen sensitivity does not directly correlate with species' lifespan.en
dc.language.isoen_USen
dc.publisherImpact Journals LLCen
dc.relation.isversionofdoi:10.18632/aging.100958en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4931838/pdf/en
dash.licenseLAAen_US
dc.subjectfibroblastsen
dc.subjectoxygenen
dc.subjectsenescenceen
dc.subjecthumanen
dc.subjectrodentsen
dc.titleSensitivity of primary fibroblasts in culture to atmospheric oxygen does not correlate with species lifespanen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalAging (Albany NY)en
dash.depositing.authorGladyshev, Vadim N.en_US
dc.date.available2016-08-09T14:52:59Z
dc.identifier.doi10.18632/aging.100958*
dash.authorsorderedfalse
dash.contributor.affiliatedGladyshev, Vadim


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