dc.contributor.author | Glykys, Joseph | en_US |
dc.contributor.author | Staley, Kevin J. | en_US |
dc.date.accessioned | 2016-08-09T14:53:02Z | |
dc.date.issued | 2016 | en_US |
dc.identifier.citation | Glykys, Joseph, and Kevin J. Staley. 2016. “Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices.” PLoS ONE 11 (6): e0158012. doi:10.1371/journal.pone.0158012. http://dx.doi.org/10.1371/journal.pone.0158012. | en |
dc.identifier.issn | 1932-6203 | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:27822232 | |
dc.description.abstract | The intraneuronal chloride concentration ([Cl-]i) is paramount for determining the polarity of signaling at GABAA synapses in the central nervous system. Sectioning hippocampal brain slices increases [Cl-]i in the superficial layers. It is not known whether cutting trauma also increases [Cl-]i in the neocortex and thalamus, and whether the effects of trauma change during development. We used Cl- imaging to study the [Cl-]i vs. the distance from the cut surface in acute thalamocortical slices from mice at developmental ages ranging from post-natal day 5 (P5) to P20. We demonstrate: 1) [Cl-]i is higher in the most superficial areas in both neocortical and thalamic brain slices at all ages tested and, 2) there is a developmental decrease in [Cl-]i that is independent of acute trauma caused by brain slicing. We conclude that [Cl-]i has a developmental progression during P5-20 in both the neocortex and thalamus. However, in both brain regions and during development the neurons closest to the slicing trauma have an elevated [Cl-]i. | en |
dc.language.iso | en_US | en |
dc.publisher | Public Library of Science | en |
dc.relation.isversionof | doi:10.1371/journal.pone.0158012 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919081/pdf/ | en |
dash.license | LAA | en_US |
dc.subject | Biology and Life Sciences | en |
dc.subject | Cell Biology | en |
dc.subject | Cellular Types | en |
dc.subject | Animal Cells | en |
dc.subject | Neurons | en |
dc.subject | Neuroscience | en |
dc.subject | Cellular Neuroscience | en |
dc.subject | Anatomy | en |
dc.subject | Brain | en |
dc.subject | Neocortex | en |
dc.subject | Medicine and Health Sciences | en |
dc.subject | Thalamus | en |
dc.subject | Neurology | en |
dc.subject | Brain Damage | en |
dc.subject | Critical Care and Emergency Medicine | en |
dc.subject | Trauma Medicine | en |
dc.subject | Developmental Neuroscience | en |
dc.subject | Specimen Preparation and Treatment | en |
dc.subject | Specimen Sectioning | en |
dc.subject | Hippocampus | en |
dc.subject | Cellular Structures and Organelles | en |
dc.subject | Extracellular Matrix | en |
dc.title | Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | PLoS ONE | en |
dash.depositing.author | Glykys, Joseph | en_US |
dc.date.available | 2016-08-09T14:53:02Z | |
dc.identifier.doi | 10.1371/journal.pone.0158012 | * |
dash.contributor.affiliated | Glykys, Joseph | |
dash.contributor.affiliated | Staley, Kevin | |