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dc.contributor.authorGlykys, Josephen_US
dc.contributor.authorStaley, Kevin J.en_US
dc.date.accessioned2016-08-09T14:53:02Z
dc.date.issued2016en_US
dc.identifier.citationGlykys, Joseph, and Kevin J. Staley. 2016. “Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices.” PLoS ONE 11 (6): e0158012. doi:10.1371/journal.pone.0158012. http://dx.doi.org/10.1371/journal.pone.0158012.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:27822232
dc.description.abstractThe intraneuronal chloride concentration ([Cl-]i) is paramount for determining the polarity of signaling at GABAA synapses in the central nervous system. Sectioning hippocampal brain slices increases [Cl-]i in the superficial layers. It is not known whether cutting trauma also increases [Cl-]i in the neocortex and thalamus, and whether the effects of trauma change during development. We used Cl- imaging to study the [Cl-]i vs. the distance from the cut surface in acute thalamocortical slices from mice at developmental ages ranging from post-natal day 5 (P5) to P20. We demonstrate: 1) [Cl-]i is higher in the most superficial areas in both neocortical and thalamic brain slices at all ages tested and, 2) there is a developmental decrease in [Cl-]i that is independent of acute trauma caused by brain slicing. We conclude that [Cl-]i has a developmental progression during P5-20 in both the neocortex and thalamus. However, in both brain regions and during development the neurons closest to the slicing trauma have an elevated [Cl-]i.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0158012en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919081/pdf/en
dash.licenseLAAen_US
dc.subjectBiology and Life Sciencesen
dc.subjectCell Biologyen
dc.subjectCellular Typesen
dc.subjectAnimal Cellsen
dc.subjectNeuronsen
dc.subjectNeuroscienceen
dc.subjectCellular Neuroscienceen
dc.subjectAnatomyen
dc.subjectBrainen
dc.subjectNeocortexen
dc.subjectMedicine and Health Sciencesen
dc.subjectThalamusen
dc.subjectNeurologyen
dc.subjectBrain Damageen
dc.subjectCritical Care and Emergency Medicineen
dc.subjectTrauma Medicineen
dc.subjectDevelopmental Neuroscienceen
dc.subjectSpecimen Preparation and Treatmenten
dc.subjectSpecimen Sectioningen
dc.subjectHippocampusen
dc.subjectCellular Structures and Organellesen
dc.subjectExtracellular Matrixen
dc.titleDevelopmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slicesen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dash.depositing.authorGlykys, Josephen_US
dc.date.available2016-08-09T14:53:02Z
dc.identifier.doi10.1371/journal.pone.0158012*
dash.contributor.affiliatedGlykys, Joseph
dash.contributor.affiliatedStaley, Kevin


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