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dc.contributor.authorShimomura, Yasuyoen_US
dc.contributor.authorSuga, Mikaen_US
dc.contributor.authorKuriyama, Naohideen_US
dc.contributor.authorNakamura, Tomoyukien_US
dc.contributor.authorSakai, Toshikazuen_US
dc.contributor.authorKato, Yuen_US
dc.contributor.authorHara, Yoshitakaen_US
dc.contributor.authorYamashita, Chizuruen_US
dc.contributor.authorNagasaki, Hiroshien_US
dc.contributor.authorKaneki, Masaoen_US
dc.contributor.authorNishida, Osamuen_US
dc.date.accessioned2016-08-09T14:53:04Z
dc.date.issued2016en_US
dc.identifier.citationShimomura, Y., M. Suga, N. Kuriyama, T. Nakamura, T. Sakai, Y. Kato, Y. Hara, et al. 2016. “Recombinant human thrombomodulin inhibits neutrophil extracellular trap formation in vitro.” Journal of Intensive Care 4 (1): 48. doi:10.1186/s40560-016-0177-9. http://dx.doi.org/10.1186/s40560-016-0177-9.en
dc.identifier.issn2052-0492en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:27822239
dc.description.abstractThe aim of this study was to investigate the effects of recombinant human-soluble thrombomodulin (rTM) on lipopolysaccharide (LPS)-induced, platelet-dependent neutrophil extracellular trap (NET) formation (NETosis). Human peripheral blood neutrophils and platelets were co-incubated with or without LPS (0.2 μg/ml) in the presence and absence of rTM (2 μg/ml). NETosis was confirmed by immunostaining and confocal microscopy. In the absence of platelets, LPS did not induce NETosis in the neutrophils. NETosis, however, was induced by LPS when neutrophils were co-cultured with platelets (64 % of neutrophils). Notably, rTM was able to fully inhibit NETosis in neutrophils cultured with platelets and in the presence of LPS. rTM did not induce NETosis in this co-culture system (p < 0.01 versus LPS in the absence of rTM). These results show that rTM can suppress LPS-induced platelet-dependent NETosis in vitro.en
dc.language.isoen_USen
dc.publisherBioMed Centralen
dc.relation.isversionofdoi:10.1186/s40560-016-0177-9en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4957921/pdf/en
dash.licenseLAAen_US
dc.subjectDisseminated intravascular coagulationen
dc.subjectInnate immunityen
dc.subjectNeutrophil extracellular trapsen
dc.subjectSepsisen
dc.subjectThrombomodulinen
dc.titleRecombinant human thrombomodulin inhibits neutrophil extracellular trap formation in vitroen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalJournal of Intensive Careen
dash.depositing.authorKuriyama, Naohideen_US
dc.date.available2016-08-09T14:53:04Z
dc.identifier.doi10.1186/s40560-016-0177-9*
dash.authorsorderedfalse
dash.contributor.affiliatedKuriyama, Naohide
dash.contributor.affiliatedKaneki, Masao


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