A new role of hindbrain boundaries as pools of neural stem/progenitor cells regulated by Sox2

DSpace/Manakin Repository

A new role of hindbrain boundaries as pools of neural stem/progenitor cells regulated by Sox2

Citable link to this page

 

 
Title: A new role of hindbrain boundaries as pools of neural stem/progenitor cells regulated by Sox2
Author: Peretz, Yuval; Eren, Noa; Kohl, Ayelet; Hen, Gideon; Yaniv, Karina; Weisinger, Karen; Cinnamon, Yuval; Sela-Donenfeld, Dalit

Note: Order does not necessarily reflect citation order of authors.

Citation: Peretz, Yuval, Noa Eren, Ayelet Kohl, Gideon Hen, Karina Yaniv, Karen Weisinger, Yuval Cinnamon, and Dalit Sela-Donenfeld. 2016. “A new role of hindbrain boundaries as pools of neural stem/progenitor cells regulated by Sox2.” BMC Biology 14 (1): 57. doi:10.1186/s12915-016-0277-y. http://dx.doi.org/10.1186/s12915-016-0277-y.
Full Text & Related Files:
Abstract: Background: Compartment boundaries are an essential developmental mechanism throughout evolution, designated to act as organizing centers and to regulate and localize differently fated cells. The hindbrain serves as a fascinating example for this phenomenon as its early development is devoted to the formation of repetitive rhombomeres and their well-defined boundaries in all vertebrates. Yet, the actual role of hindbrain boundaries remains unresolved, especially in amniotes. Results: Here, we report that hindbrain boundaries in the chick embryo consist of a subset of cells expressing the key neural stem cell (NSC) gene Sox2. These cells co-express other neural progenitor markers such as Transitin (the avian Nestin), GFAP, Pax6 and chondroitin sulfate proteoglycan. The majority of the Sox2+ cells that reside within the boundary core are slow-dividing, whereas nearer to and within rhombomeres Sox2+ cells are largely proliferating. In vivo analyses and cell tracing experiments revealed the contribution of boundary Sox2+ cells to neurons in a ventricular-to-mantle manner within the boundaries, as well as their lateral contribution to proliferating Sox2+ cells in rhombomeres. The generation of boundary-derived neurospheres from hindbrain cultures confirmed the typical NSC behavior of boundary cells as a multipotent and self-renewing Sox2+ cell population. Inhibition of Sox2 in boundaries led to enhanced and aberrant neural differentiation together with inhibition in cell-proliferation, whereas Sox2 mis-expression attenuated neurogenesis, confirming its significant function in hindbrain neuronal organization. Conclusions: Data obtained in this study deciphers a novel role of hindbrain boundaries as repetitive pools of neural stem/progenitor cells, which provide proliferating progenitors and differentiating neurons in a Sox2-dependent regulation. Electronic supplementary material The online version of this article (doi:10.1186/s12915-016-0277-y) contains supplementary material, which is available to authorized users.
Published Version: doi:10.1186/s12915-016-0277-y
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4938926/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:27822348
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters