Rapid and Permanent Neuronal Inactivation In Vivo via Subcellular Generation of Reactive Oxygen with the Use of KillerRed

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Rapid and Permanent Neuronal Inactivation In Vivo via Subcellular Generation of Reactive Oxygen with the Use of KillerRed

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Title: Rapid and Permanent Neuronal Inactivation In Vivo via Subcellular Generation of Reactive Oxygen with the Use of KillerRed
Author: Williams, Daniel C.; El Bejjani, Rachid; Ramirez, Paula Mugno; Coakley, Sean; Kim, Shin Ae; Lee, Hyewon; Wen, Quan; Samuel, Aravinthan DT; Lu, Hang; Hilliard, Massimo A.; Hammarlund, Marc

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Citation: Williams, Daniel C., Rachid El Bejjani, Paula Mugno Ramirez, Sean Coakley, Shin Ae Kim, Hyewon Lee, Quan Wen, et al. 2013. “Rapid and Permanent Neuronal Inactivation In Vivo via Subcellular Generation of Reactive Oxygen with the Use of KillerRed.” Cell Reports 5 (2) (October): 553–563. doi:10.1016/j.celrep.2013.09.023.
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Abstract: Inactivation of selected neurons in vivo can define their contribution to specific developmental outcomes, circuit functions, and behaviors. Here, we show that the optogenetic tool KillerRed selectively, rapidly, and permanently inactivates different classes of neurons in C. elegans in response to a single light stimulus, through the generation of reactive oxygen species (ROS). Ablation scales from individual neurons in single animals to multiple neurons in populations and can be applied to freely behaving animals. Using spatially restricted illumination, we demonstrate that localized KillerRed activation in either the cell body or the axon triggers neuronal degeneration and death of the targeted cell. Finally, targeting KillerRed to mitochondria results in organelle fragmentation without killing the cell, in contrast to the cell death observed when KillerRed is targeted to the plasma membrane. We expect this genetic tool to have wide-ranging applications in studies of circuit function and subcellular responses to ROS.
Published Version: doi:10.1016/j.celrep.2013.09.023
Other Sources: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3877846/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:28409887
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