Bioengineered Self-assembled Skin as an Alternative to Skin Grafts

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Bioengineered Self-assembled Skin as an Alternative to Skin Grafts

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Title: Bioengineered Self-assembled Skin as an Alternative to Skin Grafts
Author: Climov, Mihail; Medeiros, Erika; Farkash, Evan A.; Qiao, Jizeng; Rousseau, Cecile F.; Dong, Shumin; Zawadzka, Agatha; Racki, Waldemar J.; Al-Musa, Ahmad; Sachs, David H.; Randolph, Mark A.; Huang, Christene A.; Bollenbach, Thomas J.

Note: Order does not necessarily reflect citation order of authors.

Citation: Climov, M., E. Medeiros, E. A. Farkash, J. Qiao, C. F. Rousseau, S. Dong, A. Zawadzka, et al. 2016. “Bioengineered Self-assembled Skin as an Alternative to Skin Grafts.” Plastic and Reconstructive Surgery Global Open 4 (6): e731. doi:10.1097/GOX.0000000000000723. http://dx.doi.org/10.1097/GOX.0000000000000723.
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Abstract: For patients with extensive burns or donor site scarring, the limited availability of autologous and the inevitable rejection of allogeneic skin drive the need for new alternatives. Existing engineered biologic and synthetic skin analogs serve as temporary coverage until sufficient autologous skin is available. Here we report successful engraftment of a self-assembled bilayered skin construct derived from autologous skin punch biopsies in a porcine model. Dermal fibroblasts were stimulated to produce an extracellular matrix and were then seeded with epidermal progenitor cells to generate an epidermis. Autologous constructs were grafted onto partial- and full-thickness wounds. By gross examination and histology, skin construct vascularization and healing were comparable to autologous skin grafts and were superior to an autologous bilayered living cellular construct fabricated with fibroblasts cast in bovine collagen. This is the first demonstration of spontaneous vascularization and permanent engraftment of a self-assembled bilayered bioengineered skin that could supplement existing methods of reconstruction.
Published Version: doi:10.1097/GOX.0000000000000723
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4956843/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:29002493
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