Enhancer regions show high histone H3.3 turnover that changes during differentiation

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Enhancer regions show high histone H3.3 turnover that changes during differentiation

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Title: Enhancer regions show high histone H3.3 turnover that changes during differentiation
Author: Deaton, Aimee M; Gómez-Rodríguez, Mariluz; Mieczkowski, Jakub; Tolstorukov, Michael Y; Kundu, Sharmistha; Sadreyev, Ruslan I; Jansen, Lars ET; Kingston, Robert E

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Citation: Deaton, Aimee M, Mariluz Gómez-Rodríguez, Jakub Mieczkowski, Michael Y Tolstorukov, Sharmistha Kundu, Ruslan I Sadreyev, Lars ET Jansen, and Robert E Kingston. 2016. “Enhancer regions show high histone H3.3 turnover that changes during differentiation.” eLife 5 (1): e15316. doi:10.7554/eLife.15316. http://dx.doi.org/10.7554/eLife.15316.
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Abstract: The organization of DNA into chromatin is dynamic; nucleosomes are frequently displaced to facilitate the ability of regulatory proteins to access specific DNA elements. To gain insight into nucleosome dynamics, and to follow how dynamics change during differentiation, we used a technique called time-ChIP to quantitatively assess histone H3.3 turnover genome-wide during differentiation of mouse ESCs. We found that, without prior assumptions, high turnover could be used to identify regions involved in gene regulation. High turnover was seen at enhancers, as observed previously, with particularly high turnover at super-enhancers. In contrast, regions associated with the repressive Polycomb-Group showed low turnover in ESCs. Turnover correlated with DNA accessibility. Upon differentiation, numerous changes in H3.3 turnover rates were observed, the majority of which occurred at enhancers. Thus, time-ChIP measurement of histone turnover shows that active enhancers are unusually dynamic in ESCs and changes in highly dynamic nucleosomes predominate at enhancers during differentiation. DOI: http://dx.doi.org/10.7554/eLife.15316.001
Published Version: doi:10.7554/eLife.15316
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4965263/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:29002506
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