Cyclin A2 promotes DNA repair in the brain during both development and aging
Gygli, Patrick E.
Chang, Joshua C.
Gokozan, Hamza N.
Catacutan, Fay P.
Schmidt, Theresa A.
Baig, Faisal S.
Nelson, Randy J.
Otero, José J.Note: Order does not necessarily reflect citation order of authors.
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CitationGygli, P. E., J. C. Chang, H. N. Gokozan, F. P. Catacutan, T. A. Schmidt, B. Kaya, M. Goksel, et al. 2016. “Cyclin A2 promotes DNA repair in the brain during both development and aging.” Aging (Albany NY) 8 (7): 1540-1564. doi:10.18632/aging.100990. http://dx.doi.org/10.18632/aging.100990.
AbstractVarious stem cell niches of the brain have differential requirements for Cyclin A2. Cyclin A2 loss results in marked cerebellar dysmorphia, whereas forebrain growth is retarded during early embryonic development yet achieves normal size at birth. To understand the differential requirements of distinct brain regions for Cyclin A2, we utilized neuroanatomical, transgenic mouse, and mathematical modeling techniques to generate testable hypotheses that provide insight into how Cyclin A2 loss results in compensatory forebrain growth during late embryonic development. Using unbiased measurements of the forebrain stem cell niche, we parameterized a mathematical model whereby logistic growth instructs progenitor cells as to the cell-types of their progeny. Our data was consistent with prior findings that progenitors proliferate along an auto-inhibitory growth curve. The growth retardation in CCNA2-null brains corresponded to cell cycle lengthening, imposing a developmental delay. We hypothesized that Cyclin A2 regulates DNA repair and that CCNA2-null progenitors thus experienced lengthened cell cycle. We demonstrate that CCNA2-null progenitors suffer abnormal DNA repair, and implicate Cyclin A2 in double-strand break repair. Cyclin A2's DNA repair functions are conserved among cell lines, neural progenitors, and hippocampal neurons. We further demonstrate that neuronal CCNA2 ablation results in learning and memory deficits in aged mice.
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