dc.contributor.author | Tian, Bo | en_US |
dc.contributor.author | Maidana, Daniel E. | en_US |
dc.contributor.author | Dib, Bernard | en_US |
dc.contributor.author | Miller, John B. | en_US |
dc.contributor.author | Bouzika, Peggy | en_US |
dc.contributor.author | Miller, Joan W. | en_US |
dc.contributor.author | Vavvas, Demetrios G. | en_US |
dc.contributor.author | Lin, Haijiang | en_US |
dc.date.accessioned | 2016-10-11T20:27:13Z | |
dc.date.issued | 2016 | en_US |
dc.identifier.citation | Tian, Bo, Daniel E. Maidana, Bernard Dib, John B. Miller, Peggy Bouzika, Joan W. Miller, Demetrios G. Vavvas, and Haijiang Lin. 2016. “miR-17-3p Exacerbates Oxidative Damage in Human Retinal Pigment Epithelial Cells.” PLoS ONE 11 (8): e0160887. doi:10.1371/journal.pone.0160887. http://dx.doi.org/10.1371/journal.pone.0160887. | en |
dc.identifier.issn | 1932-6203 | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:29002607 | |
dc.description.abstract | Oxidative stress has been shown to contribute to the development of age-related macular degeneration (AMD). MicroRNAs (miRNA) are small non-coding RNA molecules that function in RNA silencing and post-transcriptional regulation of gene expression. We showed miR-17-3p to be elevated in macular RPE cells from AMD patients and in ARPE-19 cells under oxidative stress. Transfection of miR-17-3p mimic in ARPE-19 induced cell death and exacerbated oxidative lethality that was alleviated by miR-17-3p inhibitor. The expression of antioxidant enzymes manganese superoxide dismutase (MnSOD) and thioredoxin reductase-2 (TrxR2) were suppressed by miR-17-3p mimic and reversed by miR-17-3p inhibitor. These results suggest miR-17-3p aggravates oxidative damage-induced cell death in human RPE cells, while miR-17-3p inhibitor acts as a potential protector against oxidative stress by regulating the expression of antioxidant enzymes. | en |
dc.language.iso | en_US | en |
dc.publisher | Public Library of Science | en |
dc.relation.isversionof | doi:10.1371/journal.pone.0160887 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4978424/pdf/ | en |
dash.license | LAA | en_US |
dc.subject | Biology and Life Sciences | en |
dc.subject | Cell Biology | en |
dc.subject | Oxidative Stress | en |
dc.subject | Biochemistry | en |
dc.subject | Antioxidants | en |
dc.subject | Molecular Biology | en |
dc.subject | Molecular Biology Techniques | en |
dc.subject | Transfection | en |
dc.subject | Biology and life sciences | en |
dc.subject | Genetics | en |
dc.subject | Gene expression | en |
dc.subject | Gene regulation | en |
dc.subject | MicroRNAs | en |
dc.subject | Nucleic acids | en |
dc.subject | RNA | en |
dc.subject | Non-coding RNA | en |
dc.subject | Cell Processes | en |
dc.subject | Cell Death | en |
dc.subject | Medicine and Health Sciences | en |
dc.subject | Geriatrics | en |
dc.subject | Geriatric Ophthalmology | en |
dc.subject | Macular Degeneration | en |
dc.subject | Ophthalmology | en |
dc.subject | Retinal Disorders | en |
dc.subject | Macular Disorders | en |
dc.subject | Retinal Degeneration | en |
dc.subject | Enzymology | en |
dc.subject | Enzyme Chemistry | en |
dc.subject | Enzyme Regulation | en |
dc.subject | Anatomy | en |
dc.subject | Head | en |
dc.subject | Eyes | en |
dc.subject | Ocular System | en |
dc.title | miR-17-3p Exacerbates Oxidative Damage in Human Retinal Pigment Epithelial Cells | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | PLoS ONE | en |
dash.depositing.author | Tian, Bo | en_US |
dc.date.available | 2016-10-11T20:27:13Z | |
dc.identifier.doi | 10.1371/journal.pone.0160887 | * |
dash.authorsordered | false | |
dash.contributor.affiliated | Maidana, Daniel | |
dash.contributor.affiliated | Lin, Haijiang | |
dash.contributor.affiliated | Bouzika, Peggy | |
dash.contributor.affiliated | Tian, Bo | |
dash.contributor.affiliated | Miller, John | |
dash.contributor.affiliated | Miller, Joan | |
dash.contributor.affiliated | Vavvas, Demetrios | |