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dc.contributor.authorMichener, Joshua K.en_US
dc.contributor.authorVuilleumier, Stéphaneen_US
dc.contributor.authorBringel, Françoiseen_US
dc.contributor.authorMarx, Christopher J.en_US
dc.date.accessioned2016-10-11T20:28:26Z
dc.date.issued2016en_US
dc.identifier.citationMichener, Joshua K., Stéphane Vuilleumier, Françoise Bringel, and Christopher J. Marx. 2016. “Transfer of a Catabolic Pathway for Chloromethane in Methylobacterium Strains Highlights Different Limitations for Growth with Chloromethane or with Dichloromethane.” Frontiers in Microbiology 7 (1): 1116. doi:10.3389/fmicb.2016.01116. http://dx.doi.org/10.3389/fmicb.2016.01116.en
dc.identifier.issn1664-302Xen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:29002683
dc.description.abstractChloromethane (CM) is an ozone-depleting gas, produced predominantly from natural sources, that provides an important carbon source for microbes capable of consuming it. CM catabolism has been difficult to study owing to the challenging genetics of its native microbial hosts. Since the pathways for CM catabolism show evidence of horizontal gene transfer, we reproduced this transfer process in the laboratory to generate new CM-catabolizing strains in tractable hosts. We demonstrate that six putative accessory genes improve CM catabolism, though heterologous expression of only one of the six is strictly necessary for growth on CM. In contrast to growth of Methylobacterium strains with the closely related compound dichloromethane (DCM), we find that chloride export does not limit growth on CM and, in general that the ability of a strain to grow on DCM is uncorrelated with its ability to grow on CM. This heterologous expression system allows us to investigate the components required for effective CM catabolism and the factors that limit effective catabolism after horizontal transfer.en
dc.language.isoen_USen
dc.publisherFrontiers Media S.A.en
dc.relation.isversionofdoi:10.3389/fmicb.2016.01116en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4949252/pdf/en
dash.licenseLAAen_US
dc.subjecthorizontal gene transfer (HGT)en
dc.subjectbioremediationen
dc.subjectchloromethaneen
dc.subjecten
dc.subjectmicrobial evolutionen
dc.titleTransfer of a Catabolic Pathway for Chloromethane in Methylobacterium Strains Highlights Different Limitations for Growth with Chloromethane or with Dichloromethaneen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalFrontiers in Microbiologyen
dc.date.available2016-10-11T20:28:26Z
dc.identifier.doi10.3389/fmicb.2016.01116*


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