Coupling primary and stem cell–derived cardiomyocytes in an in vitro model of cardiac cell therapy

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Coupling primary and stem cell–derived cardiomyocytes in an in vitro model of cardiac cell therapy

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Title: Coupling primary and stem cell–derived cardiomyocytes in an in vitro model of cardiac cell therapy
Author: Aratyn-Schaus, Yvonne; Pasqualini, Francesco S.; Yuan, Hongyan; McCain, Megan L.; Ye, George J.C.; Sheehy, Sean P.; Campbell, Patrick H.; Parker, Kevin Kit

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Citation: Aratyn-Schaus, Yvonne, Francesco S. Pasqualini, Hongyan Yuan, Megan L. McCain, George J.C. Ye, Sean P. Sheehy, Patrick H. Campbell, and Kevin Kit Parker. 2016. “Coupling primary and stem cell–derived cardiomyocytes in an in vitro model of cardiac cell therapy.” The Journal of Cell Biology 212 (4): 389-397. doi:10.1083/jcb.201508026. http://dx.doi.org/10.1083/jcb.201508026.
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Abstract: The efficacy of cardiac cell therapy depends on the integration of existing and newly formed cardiomyocytes. Here, we developed a minimal in vitro model of this interface by engineering two cell microtissues (μtissues) containing mouse cardiomyocytes, representing spared myocardium after injury, and cardiomyocytes generated from embryonic and induced pluripotent stem cells, to model newly formed cells. We demonstrated that weaker stem cell–derived myocytes coupled with stronger myocytes to support synchronous contraction, but this arrangement required focal adhesion-like structures near the cell–cell junction that degrade force transmission between cells. Moreover, we developed a computational model of μtissue mechanics to demonstrate that a reduction in isometric tension is sufficient to impair force transmission across the cell–cell boundary. Together, our in vitro and in silico results suggest that mechanotransductive mechanisms may contribute to the modest functional benefits observed in cell-therapy studies by regulating the amount of contractile force effectively transmitted at the junction between newly formed and spared myocytes.
Published Version: doi:10.1083/jcb.201508026
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4754718/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:29002688
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