A magneto-DNA nanoparticle system for the rapid and sensitive diagnosis of enteric fever

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A magneto-DNA nanoparticle system for the rapid and sensitive diagnosis of enteric fever

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Title: A magneto-DNA nanoparticle system for the rapid and sensitive diagnosis of enteric fever
Author: Park, Ki Soo; Chung, Hyun Jung; Khanam, Farhana; Lee, Hakho; Rashu, Rasheduzzaman; Bhuiyan, Md. Taufiqur; Berger, Amanda; Harris, Jason B.; Calderwood, Stephen B.; Ryan, Edward T.; Qadri, Firdausi; Weissleder, Ralph; Charles, Richelle C.

Note: Order does not necessarily reflect citation order of authors.

Citation: Park, K. S., H. J. Chung, F. Khanam, H. Lee, R. Rashu, M. T. Bhuiyan, A. Berger, et al. 2016. “A magneto-DNA nanoparticle system for the rapid and sensitive diagnosis of enteric fever.” Scientific Reports 6 (1): 32878. doi:10.1038/srep32878. http://dx.doi.org/10.1038/srep32878.
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Abstract: There is currently no widely available optimal assay for diagnosing patients with enteric fever. Here we present a novel assay designed to detect amplified Salmonella nucleic acid (mRNA) using magneto-DNA probes and a miniaturized nuclear magnetic resonance device. We designed primers for genes specific to S. Typhi, S. Paratyphi A, and genes conserved among Salmonella enterica spp. and utilized strongly magnetized nanoparticles to enhance the detection signal. Blood samples spiked with in vitro grown S. Typhi, S. Paratyphi A, S. Typhimurium, and E. coli were used to confirm the specificity of each probe-set, and serial 10-fold dilutions were used to determine the limit of the detection of the assay, 0.01–1.0 CFU/ml. For proof of principle, we applied our assay to 0.5 mL blood samples from 5 patients with culture-confirmed enteric fever from Bangladesh in comparison to 3 healthy controls. We were able to detect amplified target cDNA in all 5 cases of enteric fever; no detectable signal was seen in the healthy controls. Our results suggest that a magneto-DNA nanoparticle system, with an assay time from blood collection of 3.5 hours, may be a promising platform for the rapid and culture-free diagnosis of enteric fever and non-typhoidal Salmonella bacteremia.
Published Version: doi:10.1038/srep32878
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5015101/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:29407863
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