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dc.contributor.authorHodges, Robin R.en_US
dc.contributor.authorLi, Dayuen_US
dc.contributor.authorShatos, Marie A.en_US
dc.contributor.authorBair, Jeffrey A.en_US
dc.contributor.authorLippestad, Mariten_US
dc.contributor.authorSerhan, Charles N.en_US
dc.contributor.authorDartt, Darlene A.en_US
dc.date.accessioned2016-11-18T20:47:01Z
dc.date.issued2016en_US
dc.identifier.citationHodges, Robin R., Dayu Li, Marie A. Shatos, Jeffrey A. Bair, Marit Lippestad, Charles N. Serhan, and Darlene A. Dartt. 2016. “Lipoxin A4 activates ALX/FPR2 Receptor to Regulate Conjunctival Goblet Cell Secretion.” Mucosal immunology :10.1038/mi.2016.33. doi:10.1038/mi.2016.33. http://dx.doi.org/10.1038/mi.2016.33.en
dc.identifier.issn1933-0219en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:29408305
dc.description.abstractConjunctival goblet cells play a major role in maintaining the mucous layer of the tear film under physiological conditions as well as in inflammatory diseases like dry eye and allergic conjunctivitis.. Resolution of inflammation is mediated by pro-resolution agonists such as lipoxin A4 (LXA4) that can also function under physiological conditions. The purpose of this study was to determine the actions of LXA4 on cultured rat conjunctival goblet cell mucin secretion, intracellular [Ca2+] ([Ca2+]i) and identify signaling pathways activated by LXA4. ALX/FPR was localized to goblet cells in rat conjunctiva and in cultured goblet cells. LXA4 significantly increased mucin secretion, [Ca2+]i, and ERK 1/2 activation. These functions were inhibited by ALX/FPR2 inhibitors. Stable analogs of LXA4 increased [Ca2+]i to the same extent as LXA4. Sequential addition of either LXA4 or resolvin D1 followed by the second compound decreased [Ca2+]i of the second compound compared to its initial response. LXA4 activated phospholipase C, -D, and A2 and downstream molecules protein kinase C, ERK 1/2, and Ca2+/calmodulin dependent kinase to increase mucin secretion and [Ca2+]i. We conclude that conjunctival goblet cells respond to LXA4 to maintain the homeostasis of the ocular surface and could be a novel treatment for dry eye diseases.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.1038/mi.2016.33en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063650/pdf/en
dash.licenseLAAen_US
dc.titleLipoxin A4 activates ALX/FPR2 Receptor to Regulate Conjunctival Goblet Cell Secretionen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalMucosal immunologyen
dash.depositing.authorHodges, Robin R.en_US
dc.date.available2016-11-18T20:47:01Z
dc.identifier.doi10.1038/mi.2016.33*
dash.contributor.affiliatedHodges, Robin
dash.contributor.affiliatedDartt, Darlene
dash.contributor.affiliatedSerhan, Charles


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