Bioprinting of 3D Convoluted Renal Proximal Tubules on Perfusable Chips

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Bioprinting of 3D Convoluted Renal Proximal Tubules on Perfusable Chips

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Title: Bioprinting of 3D Convoluted Renal Proximal Tubules on Perfusable Chips
Author: Homan, Kimberly A.; Kolesky, David B.; Skylar-Scott, Mark A.; Herrmann, Jessica; Obuobi, Humphrey; Moisan, Annie; Lewis, Jennifer A.

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Citation: Homan, Kimberly A., David B. Kolesky, Mark A. Skylar-Scott, Jessica Herrmann, Humphrey Obuobi, Annie Moisan, and Jennifer A. Lewis. 2016. “Bioprinting of 3D Convoluted Renal Proximal Tubules on Perfusable Chips.” Scientific Reports 6 (1): 34845. doi:10.1038/srep34845. http://dx.doi.org/10.1038/srep34845.
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Abstract: Three-dimensional models of kidney tissue that recapitulate human responses are needed for drug screening, disease modeling, and, ultimately, kidney organ engineering. Here, we report a bioprinting method for creating 3D human renal proximal tubules in vitro that are fully embedded within an extracellular matrix and housed in perfusable tissue chips, allowing them to be maintained for greater than two months. Their convoluted tubular architecture is circumscribed by proximal tubule epithelial cells and actively perfused through the open lumen. These engineered 3D proximal tubules on chip exhibit significantly enhanced epithelial morphology and functional properties relative to the same cells grown on 2D controls with or without perfusion. Upon introducing the nephrotoxin, Cyclosporine A, the epithelial barrier is disrupted in a dose-dependent manner. Our bioprinting method provides a new route for programmably fabricating advanced human kidney tissue models on demand.
Published Version: doi:10.1038/srep34845
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5057112/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:29408383
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