An In Vivo Selection System for Homing Endonuclease Activity
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CitationGruen, Mathias, Kathy Chang, Irina Serbanescu, and David R. Liu. 2002. An In Vivo Selection System for Homing Endonuclease Activity. Nucleic Acids Research 30, no. 7: e29.
AbstractHoming endonucleases are enzymes that catalyze the highly sequence-specific cleavage of DNA. We have developed an in vivo selection in Escherichia coli that links cell survival with homing endonuclease-mediated DNA cleavage activity and sequence specificity. Using this selection, wild-type and mutant variants of three homing endonucleases were characterized without requiring protein purification and in vitro analysis. This selection system may facilitate the study of sequence-specific DNA cleaving enzymes, and selections based on this work may enable the evolution of homing endonucleases with novel activities or specificities.
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