An In Vivo Selection System for Homing Endonuclease Activity

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An In Vivo Selection System for Homing Endonuclease Activity

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Title: An In Vivo Selection System for Homing Endonuclease Activity
Author: Serbanescu, Irina; Gruen, Mathias; Chang, Kathy; Liu, David

Note: Order does not necessarily reflect citation order of authors.

Citation: Gruen, Mathias, Kathy Chang, Irina Serbanescu, and David R. Liu. 2002. An In Vivo Selection System for Homing Endonuclease Activity. Nucleic Acids Research 30, no. 7: e29.
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Abstract: Homing endonucleases are enzymes that catalyze the highly sequence-specific cleavage of DNA. We have developed an in vivo selection in Escherichia coli that links cell survival with homing endonuclease-mediated DNA cleavage activity and sequence specificity. Using this selection, wild-type and mutant variants of three homing endonucleases were characterized without requiring protein purification and in vitro analysis. This selection system may facilitate the study of sequence-specific DNA cleaving enzymes, and selections based on this work may enable the evolution of homing endonucleases with novel activities or specificities.
Published Version: http://dx.doi.org/10.1093/nar/30.7.e29
Other Sources: http://evolve.harvard.edu/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:2961939
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