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dc.contributor.authorOdajima, Junkoen_US
dc.contributor.authorSaini, Siddharthen_US
dc.contributor.authorJung, Piotren_US
dc.contributor.authorNdassa-Colday, Yasmineen_US
dc.contributor.authorFicaro, Scotten_US
dc.contributor.authorGeng, Yanen_US
dc.contributor.authorMarco, Eugenioen_US
dc.contributor.authorMichowski, Wojciechen_US
dc.contributor.authorWang, Yaoyu E.en_US
dc.contributor.authorDeCaprio, James A.en_US
dc.contributor.authorLitovchick, Larisaen_US
dc.contributor.authorMarto, Jarroden_US
dc.contributor.authorSicinski, Piotren_US
dc.date.accessioned2016-12-02T15:24:19Z
dc.date.issued2016en_US
dc.identifier.citationOdajima, J., S. Saini, P. Jung, Y. Ndassa-Colday, S. Ficaro, Y. Geng, E. Marco, et al. 2016. “Proteomic Landscape of Tissue-Specific Cyclin E Functions in Vivo.” PLoS Genetics 12 (11): e1006429. doi:10.1371/journal.pgen.1006429. http://dx.doi.org/10.1371/journal.pgen.1006429.en
dc.identifier.issn1553-7390en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:29625999
dc.description.abstractE-type cyclins (cyclins E1 and E2) are components of the cell cycle machinery that has been conserved from yeast to humans. The major function of E-type cyclins is to drive cell division. It is unknown whether in addition to their ‘core’ cell cycle functions, E-type cyclins also perform unique tissue-specific roles. Here, we applied high-throughput mass spectrometric analyses of mouse organs to define the repertoire of cyclin E protein partners in vivo. We found that cyclin E interacts with distinct sets of proteins in different compartments. These cyclin E interactors are highly enriched for phosphorylation targets of cyclin E and its catalytic partner, the cyclin-dependent kinase 2 (Cdk2). Among cyclin E interactors we identified several novel tissue-specific substrates of cyclin E-Cdk2 kinase. In proliferating compartments, cyclin E-Cdk2 phosphorylates Lin proteins within the DREAM complex. In the testes, cyclin E-Cdk2 phosphorylates Mybl1 and Dmrtc2, two meiotic transcription factors that represent key regulators of spermatogenesis. In embryonic and adult brains cyclin E interacts with proteins involved in neurogenesis, while in adult brains also with proteins regulating microtubule-based processes and microtubule cytoskeleton. We also used quantitative proteomics to demonstrate re-wiring of the cyclin E interactome upon ablation of Cdk2. This approach can be used to study how protein interactome changes during development or in any pathological state such as aging or cancer.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pgen.1006429en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5102403/pdf/en
dash.licenseLAAen_US
dc.subjectBiology and Life Sciencesen
dc.subjectCell Biologyen
dc.subjectCell Processesen
dc.subjectCell Cycle and Cell Divisionen
dc.subjectCyclinsen
dc.subjectPrecipitation Techniquesen
dc.subjectImmunoprecipitationen
dc.subjectBiochemistryen
dc.subjectProteinsen
dc.subjectPost-Translational Modificationen
dc.subjectPhosphorylationen
dc.subjectAnatomyen
dc.subjectReproductive Systemen
dc.subjectGenital Anatomyen
dc.subjectTestesen
dc.subjectMedicine and Health Sciencesen
dc.subjectPhysiologyen
dc.subjectImmune Physiologyen
dc.subjectSpleenen
dc.subjectMeiosisen
dc.subjectSpermatogenesisen
dc.subjectChromosome Biologyen
dc.subjectReproductive Physiologyen
dc.subjectProtein Interactionsen
dc.titleProteomic Landscape of Tissue-Specific Cyclin E Functions in Vivoen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS Geneticsen
dash.depositing.authorGeng, Yanen_US
dc.date.available2016-12-02T15:24:19Z
dc.identifier.doi10.1371/journal.pgen.1006429*
dash.authorsorderedfalse
dash.contributor.affiliatedDeCaprio, James
dash.contributor.affiliatedMichowski, Wojciech
dash.contributor.affiliatedSicinski, Piotr
dash.contributor.affiliatedMarto, Jarrod
dash.contributor.affiliatedGeng, Yan


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