Inhibition of JCPyV infection mediated by targeted viral genome editing using CRISPR/Cas9
MetadataShow full item record
CitationChou, Yi-ying, Annabel Krupp, Campbell Kaynor, Raphaël Gaudin, Minghe Ma, Ellen Cahir-McFarland, and Tom Kirchhausen. 2016. “Inhibition of JCPyV infection mediated by targeted viral genome editing using CRISPR/Cas9.” Scientific Reports 6 (1): 36921. doi:10.1038/srep36921. http://dx.doi.org/10.1038/srep36921.
AbstractProgressive multifocal leukoencephalopathy (PML) is a debilitating disease resulting from infection of oligodendrocytes by the JC polyomavirus (JCPyV). Currently, there is no anti-viral therapeutic available against JCPyV infection. The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system (CRISPR/Cas9) is a genome editing tool capable of introducing sequence specific breaks in double stranded DNA. Here we show that the CRISPR/Cas9 system can restrict the JCPyV life cycle in cultured cells. We utilized CRISPR/Cas9 to target the noncoding control region and the late gene open reading frame of the JCPyV genome. We found significant inhibition of virus replication and viral protein expression in cells recipient of Cas9 together with JCPyV-specific single-guide RNA delivered prior to or after JCPyV infection.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:29626029
- HMS Scholarly Articles