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dc.contributor.authorKim, Bo Gyungen_US
dc.contributor.authorFujita, Takeshien_US
dc.contributor.authorStankovic, Konstantina M.en_US
dc.contributor.authorWelling, D. Bradleyen_US
dc.contributor.authorMoon, In Seoken_US
dc.contributor.authorChoi, Jae Youngen_US
dc.contributor.authorYun, Jieunen_US
dc.contributor.authorKang, Jong Soonen_US
dc.contributor.authorLee, Jong Daeen_US
dc.date.accessioned2016-12-02T15:24:52Z
dc.date.issued2016en_US
dc.identifier.citationKim, Bo Gyung, Takeshi Fujita, Konstantina M. Stankovic, D. Bradley Welling, In Seok Moon, Jae Young Choi, Jieun Yun, Jong Soon Kang, and Jong Dae Lee. 2016. “Sulforaphane, a natural component of broccoli, inhibits vestibular schwannoma growth in vitro and in vivo.” Scientific Reports 6 (1): 36215. doi:10.1038/srep36215. http://dx.doi.org/10.1038/srep36215.en
dc.identifier.issn2045-2322en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:29626096
dc.description.abstractVestibular schwannoma (VS) is an intracranial tumor that causes significant morbidity, including hearing loss, tinnitus, dizziness, and possibly even death from brainstem compression. However, FDA-approved pharmacologic treatments for VS do not exist. Sulforaphane (SFN) is a naturally occurring isothiocyanate found in cruciferous vegetables, such as broccoli, with potent chemoprotective effects in several cell types. Our objective was to determine whether SFN is effective against VS in vitro and in vivo. Human primary VS cells, HEI-193 schwannoma cells, and SC4 Nf2−/− Schwann cells were used to investigate the inhibitory effects of SFN in vitro. Cell proliferation was assessed by bromodeoxyuridine (BrdU) incorporation, and cell viability and metabolic activity was calculated by MTT assay. Apoptosis was measured by flow cytometry, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining, and Western blot for cleaved caspases. A mouse model with a murine schwannoma allograft was also used to examine the antitumor activity of SFN. SFN exhibited significant antiproliferative activity in schwannoma cells in vitro, via the inhibition of HDAC activity and the activation of ERK. SFN treatment induced apoptosis and cell cycle arrest at the G2/M phase. SFN also significantly inhibited schwannoma growth in vivo. Our preclinical studies motivate a future prospective clinical study of SFN for the treatment of VS.en
dc.language.isoen_USen
dc.publisherNature Publishing Groupen
dc.relation.isversionofdoi:10.1038/srep36215en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5090244/pdf/en
dash.licenseLAAen_US
dc.titleSulforaphane, a natural component of broccoli, inhibits vestibular schwannoma growth in vitro and in vivoen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalScientific Reportsen
dash.depositing.authorStankovic, Konstantina M.en_US
dc.date.available2016-12-02T15:24:52Z
dc.identifier.doi10.1038/srep36215*
dash.contributor.affiliatedWelling, Duane
dash.contributor.affiliatedStankovic, Konstantina


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