Comparative Analysis of Cas9 Activators Across Multiple Species

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Comparative Analysis of Cas9 Activators Across Multiple Species

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Title: Comparative Analysis of Cas9 Activators Across Multiple Species
Author: Chavez, Alejandro; Tuttle, Marcelle; Pruitt, Benjamin W; Ewen-Campen, Ben; Chari, Raj; Ter-Ovanesyan, Dmitry; Haque, Sabina J; Cecchi, Ryan J; Kowal, Emma J K; Buchthal, Joanna; Housden, Benjamin E; Perrimon, Norbert; Collins, James J; Church, George

Note: Order does not necessarily reflect citation order of authors.

Citation: Chavez, A., M. Tuttle, B. W. Pruitt, B. Ewen-Campen, R. Chari, D. Ter-Ovanesyan, S. J. Haque, et al. 2016. “Comparative Analysis of Cas9 Activators Across Multiple Species.” Nature methods 13 (7): 563-567. doi:10.1038/nmeth.3871.
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Abstract: Several groups have generated programmable transcription factors based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several human, mouse, and fly cell lines. We also explore the potential for improved activation through the combination of the most potent activator systems and assess the role of cooperativity in maximizing gene expression.
Published Version: doi:10.1038/nmeth.3871
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