NF-κB signalling and cell fate decisions in response to a short pulse of tumour necrosis factor
Lee, Robin E. C.
Qasaimeh, Mohammad A.
MetadataShow full item record
CitationLee, Robin E. C., Mohammad A. Qasaimeh, Xianfang Xia, David Juncker, and Suzanne Gaudet. 2016. “NF-κB signalling and cell fate decisions in response to a short pulse of tumour necrosis factor.” Scientific Reports 6 (1): 39519. doi:10.1038/srep39519. http://dx.doi.org/10.1038/srep39519.
AbstractIn tissues and tumours, cell behaviours are regulated by multiple time-varying signals. While in the laboratory cells are often exposed to a stimulus for the duration of the experiment, in vivo exposures may be much shorter. In this study, we monitored NF-κB and caspase signalling in human cancer cells treated with a short pulse of Tumour Necrosis Factor (TNF). TNF is an inflammatory cytokine that can induce both the pro-survival NF-κB-driven gene transcription pathway and the pro-apoptotic caspase pathway. We find that a few seconds of exposure to TNF is sufficient to activate the NF-κB pathway in HeLa cells and induce apoptotic cell death in both HeLa and Kym-1 cells. Strikingly, a 1-min pulse of TNF can be more effective at killing than a 1-hour pulse, indicating that in addition to TNF concentration, duration of exposure also coordinates cell fate decisions.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:29739022
- HMS Scholarly Articles