dc.contributor.author | Lee, Robin E. C. | en_US |
dc.contributor.author | Qasaimeh, Mohammad A. | en_US |
dc.contributor.author | Xia, Xianfang | en_US |
dc.contributor.author | Juncker, David | en_US |
dc.contributor.author | Gaudet, Suzanne | en_US |
dc.date.accessioned | 2017-01-03T23:48:59Z | |
dc.date.issued | 2016 | en_US |
dc.identifier.citation | Lee, Robin E. C., Mohammad A. Qasaimeh, Xianfang Xia, David Juncker, and Suzanne Gaudet. 2016. “NF-κB signalling and cell fate decisions in response to a short pulse of tumour necrosis factor.” Scientific Reports 6 (1): 39519. doi:10.1038/srep39519. http://dx.doi.org/10.1038/srep39519. | en |
dc.identifier.issn | 2045-2322 | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:29739022 | |
dc.description.abstract | In tissues and tumours, cell behaviours are regulated by multiple time-varying signals. While in the laboratory cells are often exposed to a stimulus for the duration of the experiment, in vivo exposures may be much shorter. In this study, we monitored NF-κB and caspase signalling in human cancer cells treated with a short pulse of Tumour Necrosis Factor (TNF). TNF is an inflammatory cytokine that can induce both the pro-survival NF-κB-driven gene transcription pathway and the pro-apoptotic caspase pathway. We find that a few seconds of exposure to TNF is sufficient to activate the NF-κB pathway in HeLa cells and induce apoptotic cell death in both HeLa and Kym-1 cells. Strikingly, a 1-min pulse of TNF can be more effective at killing than a 1-hour pulse, indicating that in addition to TNF concentration, duration of exposure also coordinates cell fate decisions. | en |
dc.language.iso | en_US | en |
dc.publisher | Nature Publishing Group | en |
dc.relation.isversionof | doi:10.1038/srep39519 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5177917/pdf/ | en |
dash.license | LAA | en_US |
dc.title | NF-κB signalling and cell fate decisions in response to a short pulse of tumour necrosis factor | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | Scientific Reports | en |
dash.depositing.author | Gaudet, Suzanne | en_US |
dc.date.available | 2017-01-03T23:48:59Z | |
dc.identifier.doi | 10.1038/srep39519 | * |
dash.contributor.affiliated | Gaudet, Suzanne | |