Hepatitis C virus NS3‐4A inhibits the peroxisomal MAVS‐dependent antiviral signalling response
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Author
Ferreira, Ana R.
Magalhães, Ana C.
Camões, Fátima
Gouveia, Ana
Vieira, Marta
Ribeiro, Daniela
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https://doi.org/10.1111/jcmm.12801Metadata
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Ferreira, Ana R., Ana C. Magalhães, Fátima Camões, Ana Gouveia, Marta Vieira, Jonathan C. Kagan, and Daniela Ribeiro. 2016. “Hepatitis C virus NS3‐4A inhibits the peroxisomal MAVS‐dependent antiviral signalling response.” Journal of Cellular and Molecular Medicine 20 (4): 750-757. doi:10.1111/jcmm.12801. http://dx.doi.org/10.1111/jcmm.12801.Abstract
Abstract Hepatitis C virus (HCV) is the cause of one of the most prevalent viral infections worldwide. Upon infection, the HCV genome activates the RIG‐I‐MAVS signalling pathway leading to the production of direct antiviral effectors which prevent important steps in viral propagation. MAVS localizes at peroxisomes and mitochondria and coordinate the activation of an effective antiviral response: peroxisomal MAVS is responsible for a rapid but short‐termed antiviral response, while the mitochondrial MAVS is associated with the activation of a stable response with delayed kinetics. The HCV NS3‐4A protease was shown to specifically cleave the mitochondrial MAVS, inhibiting the downstream response. In this study, we have analysed whether HCV NS3‐4A is also able to cleave the peroxisomal MAVS and whether this would have any effect on the cellular antiviral response. We show that NS3‐4A is indeed able to specifically cleave this protein and release it into the cytosol, a mechanism that seems to occur at a similar kinetic rate as the cleavage of the mitochondrial MAVS. Under these conditions, RIG‐I‐like receptor (RLR) signalling from peroxisomes is blocked and antiviral gene expression is inhibited. Our results also show that NS3‐4A is able to localize at peroxisomes in the absence of MAVS. However, mutation studies have shown that this localization pattern is preferred in the presence of a fully cleavable MAVS. These findings present evidence of a viral evasion strategy that disrupts RLR signalling on peroxisomes and provide an excellent example of how a single viral evasion strategy can block innate immune signalling from different organelles.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5125814/pdf/Terms of Use
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