Tailored transgene expression to specific cell types in the central nervous system after peripheral injection with AAV9

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Tailored transgene expression to specific cell types in the central nervous system after peripheral injection with AAV9

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Title: Tailored transgene expression to specific cell types in the central nervous system after peripheral injection with AAV9
Author: Dashkoff, Jonathan; Lerner, Eli P; Truong, Nhi; Klickstein, Jacob A; Fan, Zhanyun; Mu, Dakai; Maguire, Casey A; Hyman, Bradley T; Hudry, Eloise

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Citation: Dashkoff, Jonathan, Eli P Lerner, Nhi Truong, Jacob A Klickstein, Zhanyun Fan, Dakai Mu, Casey A Maguire, Bradley T Hyman, and Eloise Hudry. 2016. “Tailored transgene expression to specific cell types in the central nervous system after peripheral injection with AAV9.” Molecular Therapy. Methods & Clinical Development 3 (1): 16081. doi:10.1038/mtm.2016.81. http://dx.doi.org/10.1038/mtm.2016.81.
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Abstract: The capacity of certain adeno-associated virus (AAV) vectors to cross the blood–brain barrier after intravenous delivery offers a unique opportunity for noninvasive brain delivery. However, without a well-tailored system, the use of a peripheral route injection may lead to undesirable transgene expression in nontarget cells or organs. To refine this approach, the present study characterizes the transduction profiles of new self-complementary AAV9 (scAAV9) expressing the green fluorescent protein (GFP) either under an astrocyte (glial fibrillary acidic (GFA) protein) or neuronal (Synapsin (Syn)) promoter, after intravenous injection of adult mice (2 × 1013 vg/kg). ScAAV9-GFA-GFP and scAAV9-Syn-GFP robustly transduce astrocytes (11%) and neurons (17%), respectively, without aberrant expression leakage. Interestingly, while the percentages of GFP-positive astrocytes with scAAV9-GFA-GFP are similar to the performances observed with scAAV9-CBA-GFP (broadly active promoter), significant higher percentages of neurons express GFP with scAAV9-Syn-GFP. GFP-positive excitatory as well as inhibitory neurons are observed, as well as motor neurons in the spinal cord. Additionally, both activated (GFAP-positive) and resting astrocytes (GFAP-negative) express the reporter gene after scAAV9-GFA-GFP injection. These data thoroughly characterize the gene expression specificity of AAVs fitted with neuronal and astrocyte-selective promoters after intravenous delivery, which will prove useful for central nervous system (CNS) gene therapy approaches in which peripheral expression of transgene is a concern.
Published Version: doi:10.1038/mtm.2016.81
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5142512/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:29739075
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