Engineering Genetically-Encoded Mineralization and Magnetism via Directed Evolution

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Engineering Genetically-Encoded Mineralization and Magnetism via Directed Evolution

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Title: Engineering Genetically-Encoded Mineralization and Magnetism via Directed Evolution
Author: Liu, Xueliang; Lopez, Paola A.; Giessen, Tobias W.; Giles, Michael; Way, Jeffrey C.; Silver, Pamela A.

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Citation: Liu, Xueliang, Paola A. Lopez, Tobias W. Giessen, Michael Giles, Jeffrey C. Way, and Pamela A. Silver. 2016. “Engineering Genetically-Encoded Mineralization and Magnetism via Directed Evolution.” Scientific Reports 6 (1): 38019. doi:10.1038/srep38019. http://dx.doi.org/10.1038/srep38019.
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Abstract: Genetically encoding the synthesis of functional nanomaterials such as magnetic nanoparticles enables sensitive and non-invasive biological sensing and control. Via directed evolution of the natural iron-sequestering ferritin protein, we discovered key mutations that lead to significantly enhanced cellular magnetism, resulting in increased physical attraction of ferritin-expressing cells to magnets and increased contrast for cellular magnetic resonance imaging (MRI). The magnetic mutants further demonstrate increased iron biomineralization measured by a novel fluorescent genetic sensor for intracellular free iron. In addition, we engineered Escherichia coli cells with multiple genomic knockouts to increase cellular accumulation of various metals. Lastly to explore further protein candidates for biomagnetism, we characterized members of the DUF892 family using the iron sensor and magnetic columns, confirming their intracellular iron sequestration that results in increased cellular magnetization.
Published Version: doi:10.1038/srep38019
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126674/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:29739116
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