dc.contributor.author | Kontaridis, Maria Irene | |
dc.contributor.author | Liu, Xiangdong | |
dc.contributor.author | Zhang, Lang | |
dc.contributor.author | Bennett, Anton | |
dc.date.accessioned | 2017-02-07T20:55:55Z | |
dc.date.issued | 2001 | |
dc.identifier.citation | Kontaridis, Maria, Xiangdong Liu, Lei Zhang, and Anton M. Bennett. 2001. SHP-2 complex formation with the SHP-2 substrate-1 during C2C12 myogenesis. Journal of Cell Science 114: 2187-2198. | en_US |
dc.identifier.issn | 0021-9533 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:30203536 | |
dc.description.abstract | Myogenesis is a highly ordered process that involves the expression of muscle-specific genes, cell-cell recognition and multinucleated myotube formation. Although protein tyrosine kinases have figured prominently in myogenesis, the involvement of tyrosine phosphatases in this process is unknown. SHP-2 is an SH2 domain-containing tyrosine phosphatase, which positively regulates growth and differentiation. We show that in C2C12 myoblasts, SHP-2 becomes upregulated early on during myogenesis and associates with a 120 kDa tyrosyl-phosphorylated complex. We have identified that the 120 kDa complex consists of the SHP-2 substrate-1 (SHPS-1) and the Grb2-associated binder-1 (Gab-1). SHPS-1, but not Gab-1, undergoes tyrosyl phosphorylation and association with SHP-2 during myogenesis, the kinetics of which correlate with the expression of MyoD. Either constitutive expression or inducible activation of MyoD in 10T(1/2) fibroblasts promotes SHPS-1 tyrosyl phosphorylation and its association with SHP-2. It has been shown that p38 mitogen-activated protein kinase (MAPK) activity is required for the expression/activation of MyoD and MyoD-responsive genes. Inhibition of p38 MAPK by SB203580 in differentiating C2C12 myoblasts blocks MyoD expression, SHPS-1 tyrosyl phosphorylation and the association of SHPS-1 with SHP-2. These data suggest that SHPS-1/SHP-2 complex formation is an integral signaling component of skeletal muscle differentiation. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Company of Biologists | en_US |
dc.relation.isversionof | http://jcs.biologists.org/content/joces/114/11/2187.full.pdf | en_US |
dash.license | LAA | |
dc.subject | SHP-2 | en_US |
dc.subject | Tyrosine phosphatase | en_US |
dc.subject | Myogenesis | en_US |
dc.subject | Signalling | en_US |
dc.subject | Myoblast | en_US |
dc.subject | SHPS-1 | en_US |
dc.title | SHP-2 complex formation with the SHP-2 substrate-1 during C2C12 myogenesis | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en_US |
dc.relation.journal | Journal of Cell Science | en_US |
dash.depositing.author | Kontaridis, Maria Irene | |
dc.date.available | 2017-02-07T20:55:55Z | |
dash.contributor.affiliated | Kontaridis, Maria | |