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dc.contributor.authorEmiliani, V.
dc.contributor.authorCohen, Adam Ezra
dc.contributor.authorDeisseroth, K.
dc.contributor.authorHausser, M.
dc.date.accessioned2017-02-17T21:13:47Z
dc.date.issued2015
dc.identifier.citationEmiliani, V., A. E. Cohen, K. Deisseroth, and M. Hausser. 2015. All-Optical Interrogation of Neural Circuits. Journal of Neuroscience 35, no. 41: 13917–13926. doi:10.1523/jneurosci.2916-15.2015.en_US
dc.identifier.issn0270-6474en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:30366621
dc.description.abstractThere have been two recent revolutionary advances in neuroscience: First, genetically encoded activity sensors have brought the goal of optical detection of single action potentials in vivo within reach. Second, optogenetic actuators now allow the activity of neurons to be controlled with millisecond precision. These revolutions have now been combined, together with advanced microscopies, to allow “all-optical” readout and manipulation of activity in neural circuits with single-spike and single-neuron precision. This is a transformational advance that will open new frontiers in neuroscience research. Harnessing the power of light in the all-optical approach requires coexpression of genetically encoded activity sensors and optogenetic probes in the same neurons, as well as the ability to simultaneously target and record the light from the selected neurons. It has recently become possible to combine sensors and optical strategies that are sufficiently sensitive and cross talk free to enable single-action-potential sensitivity and precision for both readout and manipulation in the intact brain. The combination of simultaneous readout and manipulation from the same genetically defined cells will enable a wide range of new experiments as well as inspire new technologies for interacting with the brain. The advances described in this review herald a future where the traditional tools used for generations by physiologists to study and interact with the brain—stimulation and recording electrodes—can largely be replaced by light. We outline potential future developments in this field and discuss how the all-optical strategy can be applied to solve fundamental problems in neuroscience.en_US
dc.description.sponsorshipChemistry and Chemical Biologyen_US
dc.language.isoen_USen_US
dc.publisherSociety for Neuroscienceen_US
dc.relation.isversionofdoi:10.1523/JNEUROSCI.2916-15.2015en_US
dash.licenseLAA
dc.subjectoptogeneticsen_US
dc.subjectcalcium imagingen_US
dc.subjectwavefront shapingen_US
dc.subjecttwo-photon microscopyen_US
dc.subjectgenetically encoded voltage sensoren_US
dc.subjectgenetically encoded calcium sensoren_US
dc.titleAll-Optical Interrogation of Neural Circuitsen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalJournal of Neuroscienceen_US
dash.depositing.authorCohen, Adam Ezra
dc.date.available2017-02-17T21:13:47Z
dc.identifier.doi10.1523/JNEUROSCI.2916-15.2015*
dash.contributor.affiliatedCohen, Adam


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