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dc.contributor.authorChen, Baoenen_US
dc.contributor.authorZheng, Baohuien_US
dc.contributor.authorDeRan, Micaelen_US
dc.contributor.authorJarugumilli, Gopala K.en_US
dc.contributor.authorFu, Jianjunen_US
dc.contributor.authorBrooks, Yang S.en_US
dc.contributor.authorWu, Xuen_US
dc.date.accessioned2017-02-18T01:57:47Z
dc.date.issued2016en_US
dc.identifier.citationChen, Baoen, Baohui Zheng, Micael DeRan, Gopala K. Jarugumilli, Jianjun Fu, Yang S. Brooks, and Xu Wu. 2016. “ZDHHC7-Mediated S-Palmitoylation of Scribble Regulates Cell Polarity.” Nature chemical biology 12 (9): 686-693. doi:10.1038/nchembio.2119. http://dx.doi.org/10.1038/nchembio.2119.en
dc.identifier.issn1552-4450en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:30370914
dc.description.abstractScribble (SCRIB) is a tumor suppressor protein, playing critical roles in establishing and maintaining epithelial cell polarity. Paradoxically, SCRIB is frequently amplified in human cancers, however, fails to localize properly to cell-cell junctions, suggesting that mislocalization of SCRIB contributes to tumorigenesis. Using chemical reporters, here we showed that SCRIB localization is regulated by S-palmitoylation at conserved cysteine residues. The palmitoylation-deficient mutants of SCRIB are mislocalized, leading to disruption of cell polarity and loss of their tumor suppressive activities to oncogenic YAP, MAPK and PI3K/Akt pathways. We further found that ZDHHC7 is the major palmitoyl acyltransferase regulating SCRIB. Knockout of ZDHHC7 led to SCRIB mislocalization and YAP activation, and disruption of SCRIB’s suppressive activities in HRasV12-induced cell invasion. In summary, we demonstrated that ZDHHC7-mediated SCRIB palmitoylation is critical for SCRIB membrane targeting, cell polarity, and tumor suppression, providing new mechanistic insights of how dynamic protein palmitoylation regulates cell polarity and tumorigenesis.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.1038/nchembio.2119en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4990496/pdf/en
dash.licenseLAAen_US
dc.titleZDHHC7-Mediated S-Palmitoylation of Scribble Regulates Cell Polarityen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNature chemical biologyen
dash.depositing.authorChen, Baoenen_US
dc.date.available2017-02-18T01:57:47Z
dc.identifier.doi10.1038/nchembio.2119*
dash.contributor.affiliatedBrooks, Yang S.
dash.contributor.affiliatedZheng, Baohui
dash.contributor.affiliatedJarugumilli, Gopala
dash.contributor.affiliatedChen, Baoen
dash.contributor.affiliatedWu, Xu


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