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dc.contributor.authorKasianowicz, John J.
dc.contributor.authorBrandin, Eric
dc.contributor.authorBranton, Daniel
dc.contributor.authorDeamer, David W.
dc.date.accessioned2009-06-16T18:51:57Z
dc.date.issued1996
dc.identifier.citationKasianowicz, John J., Eric Brandin, Daniel Branton, and David W. Deamer. 1996. Characterization of individual polynucleotide molecules using a membrane channel. Proceedings of the National Academy of Sciences 93(24): 13770-13773.en
dc.identifier.issn0744-2831en
dc.identifier.issn0027-8424en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:3109362
dc.description.abstractWe show that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane. Because the channel diameter can accommodate only a single strand of RNA or DNA, each polymer traverses the membrane as an extended chain that partially blocks the channel. The passage of each molecule is detected as a transient decrease of ionic current whose duration is proportional to polymer length. Channel blockades can therefore be used to measure polynucleotide length. With further improvements, the method could in principle provide direct, high-speed detection of the sequence of bases in single molecules of DNA or RNA.en
dc.description.sponsorshipMolecular and Cellular Biologyen
dc.language.isoen_USen
dc.publisherNational Academy of Sciences of the United States of Americaen
dc.relation.isversionofhttp://dx.doi.org/10.1073/pnas.93.24.13770en
dc.relation.hasversionhttp://www.mcb.harvard.edu/branton/kasianowicz01.pdfen
dash.licenseLAA
dc.titleCharacterization of Individual Polynucleotide Molecules Using a Membrane Channelen
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalProceedings of the National Academy of Sciences of the United States of Americaen
dash.depositing.authorBranton, Daniel
dc.identifier.doi10.1073/pnas.93.24.13770*
dash.contributor.affiliatedBrandin, Eric Richard
dash.contributor.affiliatedBranton, Daniel


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