Unzipping Kinetics of Double-Stranded DNA in a Nanopore
Sauer-Budge, Alexis F.
Nyamwanda, Jacqueline A.
Lubensky, David K.
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CitationSauer-Budge, Alexis F., Jacqueline A. Nyamwanda, David K. Lubensky, and Daniel Branton. 2003. Unzipping kinetics of double-stranded DNA in a nanopore. Physical Review Letters 90(23): 238101.
AbstractWe studied the unzipping of single molecules of double-stranded DNA by pulling one of their two strands through a narrow protein pore. Polymerase chain reaction analysis yielded the first direct proof of DNA unzipping in such a system. The time to unzip each molecule was inferred from the ionic current signature of DNA traversal. The distribution of times to unzip under various experimental conditions fit a simple kinetic model. Using this model, we estimated the enthalpy barriers to unzipping and the effective charge of a nucleotide in the pore, which was considerably smaller than previously assumed.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:3109364
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