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dc.contributor.authorMarx, Christopher
dc.contributor.authorMiller, Jonathan A.
dc.contributor.authorChistoserdova, Ludmila
dc.contributor.authorLidstrom, Mary E.
dc.date.accessioned2009-07-01T19:26:29Z
dc.date.issued2004
dc.identifier.citationMarx, Christopher J., Jonathan A. Miller, Ludmila Chistoserdova, and Mary E. Lidstrom. 2004. Multiple formaldehyde oxidation/detoxification pathways in Burkholderia fungorum LB400. Journal of Bacteriology 186, no. 7: 2173-2178.en
dc.identifier.issn1098-5530en
dc.identifier.issn0021-9193en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:3137805
dc.description.abstract<i>Burkholderia</i> species are free-living bacteria with a versatile metabolic lifestyle. The genome of <i>B. fungorum</i> LB400 is predicted to encode three different pathways for formaldehyde oxidation: an NAD-linked, glutathione (GSH) -independent formaldehyde dehydrogenase; an NAD-linked, GSH-dependent formaldehyde oxidation system; and a tetrahydromethanopterin-methanofuran-dependent formaldehyde oxidation system. The other <i>Burkholderia</i> species for which genome sequences are available, <i>B. mallei, B. pseudomallei</i>, and <i>B. cepacia</i>, are predicted to contain only the first two of these pathways. The roles of the three putative formaldehyde oxidation pathways in B.fungorum LB400 have been assessed via knockout mutations in each of these pathways, as well as in all combinations of knockouts. The resulting mutants have the expected loss of enzyme activities and exhibit defects of varying degrees of severity during growth on choline, a formaldehyde-producing substrate. Our data suggest that all three pathways are involved in formaldehyde detoxification and are functionally redundant under the tested conditions.en
dc.description.sponsorshipOrganismic and Evolutionary Biologyen
dc.language.isoen_USen
dc.publisherAmerican Society for Microbiologyen
dc.relation.isversionofhttp://dx.doi.org/10.1128/JB.186.7.2173-2178.2004en
dc.relation.hasversionhttp://www.oeb.harvard.edu/faculty/marx/marxlabFrameset.htmen
dash.licenseMETA_ONLY
dc.titleMultiple Formaldehyde Oxidation/Detoxification Pathways in Burkholderia Fungorum LB400en
dc.relation.journalJournal of Bacteriologyen
dash.depositing.authorMarx, Christopher
dash.embargo.until10000-01-01
dc.identifier.doi10.1128/JB.186.7.2173-2178.2004*
dash.contributor.affiliatedMarx, Christopher J


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