dc.contributor.author | Cui, Ye | en_US |
dc.contributor.author | Yu, Huansha | en_US |
dc.contributor.author | Zheng, Xin | en_US |
dc.contributor.author | Peng, Rui | en_US |
dc.contributor.author | Wang, Qiang | en_US |
dc.contributor.author | Zhou, Yi | en_US |
dc.contributor.author | Wang, Rui | en_US |
dc.contributor.author | Wang, Jiehua | en_US |
dc.contributor.author | Qu, Bo | en_US |
dc.contributor.author | Shen, Nan | en_US |
dc.contributor.author | Guo, Qiang | en_US |
dc.contributor.author | Liu, Xing | en_US |
dc.contributor.author | Wang, Chen | en_US |
dc.date.accessioned | 2017-03-28T23:47:57Z | |
dc.date.issued | 2017 | en_US |
dc.identifier.citation | Cui, Y., H. Yu, X. Zheng, R. Peng, Q. Wang, Y. Zhou, R. Wang, et al. 2017. “SENP7 Potentiates cGAS Activation by Relieving SUMO-Mediated Inhibition of Cytosolic DNA Sensing.” PLoS Pathogens 13 (1): e1006156. doi:10.1371/journal.ppat.1006156. http://dx.doi.org/10.1371/journal.ppat.1006156. | en |
dc.identifier.issn | | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:31731637 | |
dc.description.abstract | Cyclic GMP-AMP (cGAMP) synthase (cGAS, a.k.a. MB21D1), a cytosolic DNA sensor, catalyzes formation of the second messenger 2’3’-cGAMP that activates the stimulator of interferon genes (STING) signaling. How the cGAS activity is modulated remains largely unknown. Here, we demonstrate that sentrin/SUMO-specific protease 7 (SENP7) interacted with and potentiated cGAS activation. The small ubiquitin-like modifier (SUMO) was conjugated onto the lysine residues 335, 372 and 382 of cGAS, which suppressed its DNA-binding, oligomerization and nucleotidyl-transferase activities. SENP7 reversed this inhibition via catalyzing the cGAS de-SUMOylation. Consistently, silencing of SENP7 markedly impaired the IRF3-responsive gene expression induced by cGAS-STING axis. SENP7-knockdown mice were more susceptible to herpes simplex virus 1 (HSV-1) infection. SENP7 was significantly up-regulated in patients with SLE. Our study highlights the temporal modulation of the cGAS activity via dynamic SUMOylation, uncovering a novel mechanism for fine-tuning the STING signaling in innate immunity. | en |
dc.language.iso | en_US | en |
dc.publisher | Public Library of Science | en |
dc.relation.isversionof | doi:10.1371/journal.ppat.1006156 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5271409/pdf/ | en |
dash.license | LAA | en_US |
dc.subject | Biology and life sciences | en |
dc.subject | Biochemistry | en |
dc.subject | Proteins | en |
dc.subject | Post-translational modification | en |
dc.subject | SUMOylation | en |
dc.subject | Precipitation Techniques | en |
dc.subject | Immunoprecipitation | en |
dc.subject | Genetics | en |
dc.subject | Gene expression | en |
dc.subject | Gene regulation | en |
dc.subject | Small interfering RNAs | en |
dc.subject | Nucleic acids | en |
dc.subject | RNA | en |
dc.subject | Non-coding RNA | en |
dc.subject | Physical Sciences | en |
dc.subject | Chemistry | en |
dc.subject | Chemical Compounds | en |
dc.subject | Organic Compounds | en |
dc.subject | Amino Acids | en |
dc.subject | Basic Amino Acids | en |
dc.subject | Lysine | en |
dc.subject | Organic Chemistry | en |
dc.subject | Biology and Life Sciences | en |
dc.subject | Immunologic Techniques | en |
dc.subject | Immunoassays | en |
dc.subject | Enzyme-Linked Immunoassays | en |
dc.subject | Physiology | en |
dc.subject | Immune Physiology | en |
dc.subject | Antibodies | en |
dc.subject | Medicine and Health Sciences | en |
dc.subject | Immunology | en |
dc.subject | Immune System Proteins | en |
dc.subject | Spectrum Analysis Techniques | en |
dc.subject | Spectrophotometry | en |
dc.subject | Fluorophotometry | en |
dc.subject | Fluorescence Resonance Energy Transfer | en |
dc.subject | Interferons | en |
dc.title | SENP7 Potentiates cGAS Activation by Relieving SUMO-Mediated Inhibition of Cytosolic DNA Sensing | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | PLoS Pathogens | en |
dash.depositing.author | Liu, Xing | en_US |
dc.date.available | 2017-03-28T23:47:57Z | |
dc.identifier.doi | 10.1371/journal.ppat.1006156 | * |
dash.authorsordered | false | |
dash.contributor.affiliated | Liu, Xing | |