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dc.contributor.authorYang, Jun-Moen_US
dc.contributor.authorLee, Hye Shinen_US
dc.contributor.authorSeo, Ji Haeen_US
dc.contributor.authorPark, Ji-Hyeonen_US
dc.contributor.authorGelman, Irwin H.en_US
dc.contributor.authorLo, Eng H.en_US
dc.contributor.authorKim, Kyu-Wonen_US
dc.date.accessioned2017-03-28T23:48:46Z
dc.date.issued2017en_US
dc.identifier.citationYang, Jun-Mo, Hye Shin Lee, Ji Hae Seo, Ji-Hyeon Park, Irwin H. Gelman, Eng H. Lo, and Kyu-Won Kim. 2017. “Structural environment built by AKAP12+ colon mesenchymal cells drives M2 macrophages during inflammation recovery.” Scientific Reports 7 (1): 42723. doi:10.1038/srep42723. http://dx.doi.org/10.1038/srep42723.en
dc.identifier.issnen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:31731695
dc.description.abstractMacrophages exhibit phenotypic plasticity, as they have the ability to switch their functional phenotypes during inflammation and recovery. Simultaneously, the mechanical environment actively changes. However, how these dynamic alterations affect the macrophage phenotype is unknown. Here, we observed that the extracellular matrix (ECM) constructed by AKAP12+ colon mesenchymal cells (CMCs) generated M2 macrophages by regulating their shape during recovery. Notably, rounded macrophages were present in the linear and loose ECM of inflamed colons and polarized to the M1 phenotype. In contrast, ramified macrophages emerged in the contracted ECM of recovering colons and mainly expressed M2 macrophage markers. These contracted structures were not observed in the inflamed colons of AKAP12 knockout (KO) mice. Consequently, the proportion of M2 macrophages in inflamed colons was lower in AKAP12 KO mice than in WT mice. In addition, clinical symptoms and histological damage were more severe in AKAP12 KO mice than in WT mice. In experimentally remodeled collagen gels, WT CMCs drove the formation of a more compacted structure than AKAP12 KO CMCs, which promoted the polarization of macrophages toward an M2 phenotype. These results demonstrated that tissue contraction during recovery provides macrophages with the physical cues that drive M2 polarization.en
dc.language.isoen_USen
dc.publisherNature Publishing Groupen
dc.relation.isversionofdoi:10.1038/srep42723en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5311874/pdf/en
dash.licenseLAAen_US
dc.titleStructural environment built by AKAP12+ colon mesenchymal cells drives M2 macrophages during inflammation recoveryen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalScientific Reportsen
dash.depositing.authorLo, Eng H.en_US
dc.date.available2017-03-28T23:48:46Z
dc.identifier.doi10.1038/srep42723*
dash.contributor.affiliatedLo, Eng


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