Show simple item record

dc.contributor.authorKim, Soohongen_US
dc.contributor.authorDe Jonghe, Joachimen_US
dc.contributor.authorKulesa, Anthony B.en_US
dc.contributor.authorFeldman, Daviden_US
dc.contributor.authorVatanen, Tommien_US
dc.contributor.authorBhattacharyya, Roby P.en_US
dc.contributor.authorBerdy, Brittanyen_US
dc.contributor.authorGomez, Jamesen_US
dc.contributor.authorNolan, Jillen_US
dc.contributor.authorEpstein, Slavaen_US
dc.contributor.authorBlainey, Paul C.en_US
dc.date.accessioned2017-03-28T23:50:19Z
dc.date.issued2017en_US
dc.identifier.citationKim, S., J. De Jonghe, A. B. Kulesa, D. Feldman, T. Vatanen, R. P. Bhattacharyya, B. Berdy, et al. 2017. “High-throughput automated microfluidic sample preparation for accurate microbial genomics.” Nature Communications 8 (1): 13919. doi:10.1038/ncomms13919. http://dx.doi.org/10.1038/ncomms13919.en
dc.identifier.issnen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:31731799
dc.description.abstractLow-cost shotgun DNA sequencing is transforming the microbial sciences. Sequencing instruments are so effective that sample preparation is now the key limiting factor. Here, we introduce a microfluidic sample preparation platform that integrates the key steps in cells to sequence library sample preparation for up to 96 samples and reduces DNA input requirements 100-fold while maintaining or improving data quality. The general-purpose microarchitecture we demonstrate supports workflows with arbitrary numbers of reaction and clean-up or capture steps. By reducing the sample quantity requirements, we enabled low-input (∼10,000 cells) whole-genome shotgun (WGS) sequencing of Mycobacterium tuberculosis and soil micro-colonies with superior results. We also leveraged the enhanced throughput to sequence ∼400 clinical Pseudomonas aeruginosa libraries and demonstrate excellent single-nucleotide polymorphism detection performance that explained phenotypically observed antibiotic resistance. Fully-integrated lab-on-chip sample preparation overcomes technical barriers to enable broader deployment of genomics across many basic research and translational applications.en
dc.language.isoen_USen
dc.publisherNature Publishing Groupen
dc.relation.isversionofdoi:10.1038/ncomms13919en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5290157/pdf/en
dash.licenseLAAen_US
dc.titleHigh-throughput automated microfluidic sample preparation for accurate microbial genomicsen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNature Communicationsen
dash.depositing.authorBhattacharyya, Roby P.en_US
dc.date.available2017-03-28T23:50:19Z
dc.identifier.doi10.1038/ncomms13919*
dash.authorsorderedfalse
dash.contributor.affiliatedBhattacharyya, Roby


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record