Hsp65-Producing Lactococcus lactis Prevents Inflammatory Intestinal Disease in Mice by IL-10- and TLR2-Dependent Pathways
Gomes-Santos, Ana Cristina
de Oliveira, Rafael Pires
Moreira, Thaís Garcias
Castro-Junior, Archimedes Barbosa
Horta, Bernardo Coelho
de Almeida, Leonardo Augusto
Cara, Denise Carmona
Oliveira, Sérgio Costa
Azevedo, Vasco Ariston Carvalho
Faria, Ana Maria CaetanoNote: Order does not necessarily reflect citation order of authors.
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CitationGomes-Santos, A. C., R. P. de Oliveira, T. G. Moreira, A. B. Castro-Junior, B. C. Horta, L. Lemos, L. A. de Almeida, et al. 2017. “Hsp65-Producing Lactococcus lactis Prevents Inflammatory Intestinal Disease in Mice by IL-10- and TLR2-Dependent Pathways.” Frontiers in Immunology 8 (1): 30. doi:10.3389/fimmu.2017.00030. http://dx.doi.org/10.3389/fimmu.2017.00030.
AbstractHeat shock proteins (Hsps) are highly expressed at all sites of inflammation. As they are ubiquitous and immunodominant antigens, these molecules represent good candidates for the therapeutic use of oral tolerance in autoimmune and chronic inflammatory diseases. Evidences from human and animal studies indicate that inflammatory bowel disease (IBD) results from uncontrolled inflammatory responses to intestinal microbiota. Hsps are immunodominant proteins expressed by several immune cells and by commensal bacteria. Using an IBD mouse model, we showed that oral pretreatment with genetically modified Lactococcus lactis that produces and releases Mycobacterium Hsp65, completely prevented DSS-induced colitis in C57BL/6 mice. Protection was associated with reduced pro-inflammatory cytokines, such as IFN-γ, IL-6, and TNF-α; increased IL-10 production in colonic tissue; and expansion of CD4+Foxp3+ and CD4+LAP+ regulatory T cells in spleen and mesenteric lymph nodes. This effect was dependent on IL-10 and toll-like receptor 2. Thus, this approach may open alternative options for long-term management of IBD.
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