eUnaG: a new ligand-inducible fluorescent reporter to detect drug transporter activity in live cells

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eUnaG: a new ligand-inducible fluorescent reporter to detect drug transporter activity in live cells

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Title: eUnaG: a new ligand-inducible fluorescent reporter to detect drug transporter activity in live cells
Author: Yeh, Johannes T.-H.; Nam, Kwangho; Yeh, Joshua T.-H.; Perrimon, Norbert

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Citation: Yeh, Johannes T.-H., Kwangho Nam, Joshua T.-H. Yeh, and Norbert Perrimon. 2017. “eUnaG: a new ligand-inducible fluorescent reporter to detect drug transporter activity in live cells.” Scientific Reports 7 (1): 41619. doi:10.1038/srep41619. http://dx.doi.org/10.1038/srep41619.
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Abstract: The absorption, distribution, metabolism and excretion (ADME) of metabolites and toxic organic solutes are orchestrated by the ATP-binding cassette (ABC) transporters and the organic solute carrier family (SLC) proteins. A large number of ABC and SLC transpoters exist; however, only a small number have been well characterized. To facilitate the analysis of these transporters, which is important for drug safety and physiological studies, we developed a sensitive genetically encoded bilirubin (BR)-inducible fluorescence sensor (eUnaG) to detect transporter-coupled influx/efflux of organic compounds. This sensor can be used in live cells to measure transporter activity, as excretion of BR depends on ABC and SLC transporters. Applying eUnaG in functional RNAi screens, we characterize l(2)03659 as a Drosophila multidrug resistant-associated ABC transporter.
Published Version: doi:10.1038/srep41619
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5296874/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:31731812
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