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Ribosomal mutations promote the evolution of antibiotic resistance in a multidrug environment

 
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Author
Gomez, James EHARVARD
Kaufmann-Malaga, Benjamin B
Wivagg, Carl NHARVARD
Kim, Peter B
Silvis, Melanie R
Renedo, Nikolai
Ioerger, Thomas R
Ahmad, Rushdy
Livny, Jonathan
Fishbein, SkyeHARVARD
Sacchettini, James C
Carr, Steven A
Hung, Deborah THARVARD
Note: Order does not necessarily reflect citation order of authors.
Published Version
https://doi.org/10.7554/eLife.20420
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Citation
Gomez, J. E., B. B. Kaufmann-Malaga, C. N. Wivagg, P. B. Kim, M. R. Silvis, N. Renedo, T. R. Ioerger, et al. 2017. “Ribosomal mutations promote the evolution of antibiotic resistance in a multidrug environment.” eLife 6 (1): e20420. doi:10.7554/eLife.20420. http://dx.doi.org/10.7554/eLife.20420.
Abstract
Antibiotic resistance arising via chromosomal mutations is typically specific to a particular antibiotic or class of antibiotics. We have identified mutations in genes encoding ribosomal components in Mycobacterium smegmatis that confer resistance to several structurally and mechanistically unrelated classes of antibiotics and enhance survival following heat shock and membrane stress. These mutations affect ribosome assembly and cause large-scale transcriptomic and proteomic changes, including the downregulation of the catalase KatG, an activating enzyme required for isoniazid sensitivity, and upregulation of WhiB7, a transcription factor involved in innate antibiotic resistance. Importantly, while these ribosomal mutations have a fitness cost in antibiotic-free medium, in a multidrug environment they promote the evolution of high-level, target-based resistance. Further, suppressor mutations can then be easily acquired to restore wild-type growth. Thus, ribosomal mutations can serve as stepping-stones in an evolutionary path leading to the emergence of high-level, multidrug resistance. DOI: http://dx.doi.org/10.7554/eLife.20420.001
Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5319836/pdf/
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This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:31731855

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