Detecting Single Stranded DNA with a Solid State Nanopore
McNabb, David S.
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CitationFologea, Daniel, Marc Gershow, Bradley Ledden, David S. McNabb, Jene A. Golovchenko, and Jiali Li. 2005. “Detecting Single Stranded DNA with a Solid State Nanopore.” Nano Lett. 5 (10) (October): 1905–1909. doi:10.1021/nl051199m.
AbstractVoltage biased solid-state nanopores are used to detect and characterize individual single stranded DNA molecules of fixed micrometer length by operating a nanopore detector at pH values greater than ∼11.6. The distribution of observed molecular event durations and blockade currents shows that a significant fraction of the events obey a rule of constant event charge deficit (ecd) indicating that they correspond to molecules translocating through the nanopore in a distribution of folded and unfolded configurations. A surprisingly large component is unfolded. The result is an important milestone in developing solid-state nanopores for single molecule sequencing applications.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:31887692
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